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    Lines of Evidence for Horizontal Gene Transfer of a Phenazine Producing Operon into Multiple Bacterial Species


    Fitzpatrick, David A. (2009) Lines of Evidence for Horizontal Gene Transfer of a Phenazine Producing Operon into Multiple Bacterial Species. Journal of Molecular Evolution, 68 (2). pp. 171-185. ISSN 0022-2844

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    Abstract

    Phenazines are secondary metabolites with broad-spectrum antibiotic activity against bacteria, fungi, and eukaryotes. In pseudomonad species, a conserved seven-gene phenazine operon (phzABCDEFG) is required for the conversion of chorismic acid to the broad-spectrum antibiotic phenazine-1-carboxylate. Previous analyses of genes involved in phenazine production from nonpseudomonad species uncovered a high degree of sequence similarity to pseudomonad homologues. The analyses undertaken in this study wished to eluciadate the evolutionary history of genes involved in the production of phenazines. Furthermore, I wanted to determine if the phenazine operon has been transferred through horizontal gene transfer. Analyses of GC content, codon usage patterns, frequency of 3:1 dinucleotides, sequence similarities, and phylogenetic reconstructions were undertaken to map the evolutionary history of phenazine genes from multiple bacterial species. Patchy phyletic distribution, high sequence similarities, and phylogenetic evidence infer that pseudomonad, Streptomyces cinnamonensis, Pantoea agglomerans, Burkholderia cepacia, Pectobacterium atrosepticum, Brevibacterium linens, and Mycobacterium abscessus species all contain a phenazine operon which has most likely been transferred among these species through horizontal gene transfer. The acquisition of an antibiotic-associated operon is significant, as it may increase the relative fitness of the recipient species.

    Item Type: Article
    Keywords: Horizontal gene transfer; Phenazines; Phenazine operon; GC content; Phylogenetic analysis; 3:1 dinucleotide frequency;
    Academic Unit: Faculty of Science and Engineering > Biology
    Item ID: 7445
    Identification Number: https://doi.org/10.1007/s00239-009-9198-5
    Depositing User: David Fitzpatrick
    Date Deposited: 15 Sep 2016 09:54
    Journal or Publication Title: Journal of Molecular Evolution
    Publisher: Springer Verlag
    Refereed: Yes
    Funders: Health Research Board (HRB)
    URI:

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