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    Novel primers for the amplification of nuclear DNA introns in the entomopathogenic nematode Heterorhabditis bacteriophora and their cross-amplification in seven other Heterorhabditis species


    Regeai, S.O. and Dolan, K.M. and Fitzpatrick, David A. and Browne, J.A. and Jones, J.T. and Burnell, Ann (2009) Novel primers for the amplification of nuclear DNA introns in the entomopathogenic nematode Heterorhabditis bacteriophora and their cross-amplification in seven other Heterorhabditis species. Molecular Ecology Resources, 9 (1). pp. 421-424. ISSN 1755-098X

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    Abstract

    We describe 24 novel primers that amplify intron regions in housekeeping and structural genes of Heterorhabditis bacteriophora. The cross-amplification potential of these primers in seven other Heterorhabditis species was determined. The results obtained showed interspecific nucleotide, length and splice site variability in the sequenced introns and for one gene, an intron gain was observed. These primers will be useful tools for studying population genetics, genetic diversity and intron DNA evolution within the genus Heterorhabditis and other genera of rhabditid nematodes.

    Item Type: Article
    Keywords: entomopathogenic; EPIC-PCR; Heterorhabditis; intron; molecular markers; nematode;
    Academic Unit: Faculty of Science and Engineering > Biology
    Item ID: 7446
    Identification Number: https://doi.org/10.1111/j.1755-0998.2008.02431.x
    Depositing User: David Fitzpatrick
    Date Deposited: 15 Sep 2016 09:56
    Journal or Publication Title: Molecular Ecology Resources
    Publisher: Blackwell Publishing Ltd.
    Refereed: Yes
    URI:

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