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    Comparison of two DNA microarrays for detection of plasmid-mediated antimicrobial resistance and virulence factor genes in clinical isolates of Enterobacteriaceae and non-Enterobacteriaceae


    Walsh, Fiona and Cooke, Niamh M. and Smith, Stephen G. and Moran, Gary P. and Cooke, Fiona J. and Ivens, Alasdair and Wain, John and Rogers, Thomas R. (2010) Comparison of two DNA microarrays for detection of plasmid-mediated antimicrobial resistance and virulence factor genes in clinical isolates of Enterobacteriaceae and non-Enterobacteriaceae. International Journal of Antimicrobial Agents, 35 (6). pp. 593-598. ISSN 0924-8579

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    Abstract

    A DNA microarray was developed to detect plasmid-mediated antimicrobial resistance (AR) and virulence factor (VF) genes in clinical isolates of Enterobacteriaceae and non-Enterobacteriaceae. The array was validated with the following bacterial species: Escherichiacoli (n=17); Klebsiellapneumoniae (n=3); Enterobacter spp. (n=6); Acinetobacter genospecies 3 (n=1); Acinetobacterbaumannii (n=1); Pseudomonasaeruginosa (n=2); and Stenotrophomonasmaltophilia (n=2). The AR gene profiles of these isolates were identified by polymerase chain reaction (PCR). The DNA microarray consisted of 155 and 133 AR and VF gene probes, respectively. Results were compared with the commercially available Identibac AMR-ve Array Tube. Hybridisation results indicated that there was excellent correlation between PCR and array results for AR and VF genes. Genes conferring resistance to each antibiotic class were identified by the DNA array. Unusual resistance genes were also identified, such as bla(SHV-5) in a bla(OXA-23)-positive carbapenem-resistant A. baumannii. The phylogenetic group of each E. coli isolate was verified by the array. These data demonstrate that it is possible to screen simultaneously for all important classes of mobile AR and VF genes in Enterobacteriaceae and non-Enterobacteriaceae whilst also assigning a correct phylogenetic group to E. coli isolates. Therefore, it is feasible to test clinical Gram-negative bacteria for all known AR genes and to provide important information regarding pathogenicity simultaneously.

    Item Type: Article
    Keywords: β-Lactamases; Quinolones; Virulence; Molecular detection;
    Academic Unit: Faculty of Science and Engineering > Biology
    Item ID: 7476
    Identification Number: https://doi.org/10.1016/j.ijantimicag.2010.02.011
    Depositing User: Fiona Walsh
    Date Deposited: 28 Sep 2016 15:30
    Journal or Publication Title: International Journal of Antimicrobial Agents
    Publisher: Elsevier
    Refereed: Yes
    URI:

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