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    Molecular characterization of carbapenem-resistant Acinetobacter species in an Irish university hospital: predominance of Acinetobacter genomic species 3


    Boo, T.W. and Walsh, Fiona and Crowley, B. (2009) Molecular characterization of carbapenem-resistant Acinetobacter species in an Irish university hospital: predominance of Acinetobacter genomic species 3. Journal of Medical Microbiology, 58 (2). pp. 209-216. ISSN 0022-2615

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    Abstract

    A 30 month prospective study of Acinetobacter species encountered in the Central Pathology Laboratory of St James's Hospital, Dublin, Ireland, was conducted to investigate the prevalence and molecular epidemiology of carbapenem resistance in such isolates. Acinetobacter genomic species 3 (AG3) was found to be the predominant Acinetobacter species (45/114, 39 %) in our institution. A total of 11 % of all Acinetobacter species (12/114) and 22 % of AG3 isolates (10/45) were carbapenem resistant. Carbapenem resistance was mediated by Ambler class D beta-lactamase OXA-23 in all 12 isolates, with insertion sequence ISAba1 found upstream of bla(OXA-23). ISAba1 was also found upstream of bla(ADC-25), which encodes the enzyme AmpC, in an Acinetobacter baumannii isolate, and upstream of the aminoglycoside-acetyltransferase-encoding gene aacC2 in three AG3 isolates. Inter-species plasmidic transfer was most likely involved in the emergence and spread of bla(OXA-23) among the Acinetobacter isolates within our institution. The emergence of carbapenem resistance was associated not only with prior carbapenem use but also with the use of other antimicrobial agents, most notably beta-lactam/beta-lactamase-inhibitor combinations. The study demonstrated the emerging trend of carbapenem resistance in the wider context of the Acinetobacter genus, and reiterated the paramount importance of the prudent use of antimicrobial agents, stringent infection control measures and resistance surveillance of pathogens.

    Item Type: Article
    Keywords: MBL; metallo β-lactamase; ICU; intensive care unit; CLSI; Clinical and Laboratory Standards Institute; AG3; Acinetobacter genomic species 3;
    Academic Unit: Faculty of Science and Engineering > Biology
    Item ID: 7477
    Identification Number: 0.1099/jmm.0.004911-0.
    Depositing User: Fiona Walsh
    Date Deposited: 28 Sep 2016 15:30
    Journal or Publication Title: Journal of Medical Microbiology
    Publisher: Society for General Microbiology
    Refereed: Yes
    Funders: Health Research Board (HRB)
    URI:

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