MURAL - Maynooth University Research Archive Library



    Detection and molecular characterisation of plasmidic AmpC b-lactamases in Klebsiella pneumoniae isolates from a tertiary-care hospital in Dublin, Ireland


    Roche, C. and Boo, T.W. and Walsh, Fiona and Crowley, B. (2008) Detection and molecular characterisation of plasmidic AmpC b-lactamases in Klebsiella pneumoniae isolates from a tertiary-care hospital in Dublin, Ireland. Clinical Microbiology and Infection, 14 (6). pp. 616-618. ISSN 1198-743X

    [img]
    Preview
    Download (66kB) | Preview


    Share your research

    Twitter Facebook LinkedIn GooglePlus Email more...



    Add this article to your Mendeley library


    Abstract

    This study determined the types of AmpC enzymes produced by Klebsiella pneumoniae isolates resistant to third-generation cephalosporins and the clonality of these isolates. The presence of AmpC enzymes was identified by cephalosporin-cloxacillin synergy tests. Genes encoding AmpC enzymes were characterised by PCR and sequencing. Pulsed-field gel electrophoresis (PFGE) was used to type the isolates. Fifteen K. pneumoniae isolates were positive for bla(AmpC), 13 were positive for bla(ACC-1) and two were positive for bla(DHA-1). Production of the DHA-1 enzyme was inducible. The ampR gene was identified upstream of the bla(DHA-1) gene. PFGE demonstrated the polyclonal origin of the isolates carrying bla(ACC-1).

    Item Type: Article
    Keywords: ACC-1; AmpC b-lactamase; DHA-1; Klebsiella pneumoniae; resistance; typing;
    Academic Unit: Faculty of Science and Engineering > Biology
    Item ID: 7478
    Identification Number: https://doi.org/10.1111/j.1469-0691.2008.01998.x
    Depositing User: Fiona Walsh
    Date Deposited: 28 Sep 2016 15:29
    Journal or Publication Title: Clinical Microbiology and Infection
    Publisher: Elsevier
    Refereed: Yes
    Funders: Health Research Board (HRB)
    URI:

    Repository Staff Only(login required)

    View Item Item control page

    Downloads

    Downloads per month over past year