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    Streptomycin use in apple orchards did not increase abundance of mobile resistance genes


    Duffy, Brion and Holliger, E. and Walsh, Fiona (2014) Streptomycin use in apple orchards did not increase abundance of mobile resistance genes. FEMS Microbiology Letters, 350. pp. 180-189. ISSN 0378-1097

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    Abstract

    Streptomycin is used as a first-line defense and tetracycline as a second-line defense, in the fight against fire blight disease in apple and pear orchards. We have performed the first study to quantitatively analyze the influence of streptomycin use in agriculture on the abundance of streptomycin and tetracycline resistance genes in apple orchards. Flowers, leaves, and soil were collected from three orchard sites in 2010, 2011, and 2012. Gene abundance distribution was analyzed using two-way ANOVA and principal component analysis to investigate relationships between gene abundance data over time and treatment. The mobile antibiotic resistance genes, strA, strB, tetB, tetM, tetW, and the insertion sequence IS1133, were detected prior to streptomycin treatment in almost all samples, indicating the natural presence of these resistance genes in nature. Statistically significant increases in the resistance gene abundances were occasional, inconsistent, and not reproducible from one year to the next. We conclude that the application of streptomycin in these orchards was not associated with sustained increases in streptomycin or tetracycline resistance gene abundances.

    Item Type: Article
    Keywords: tetracycline; qPCR; relative quantity;
    Academic Unit: Faculty of Science and Engineering > Biology
    Item ID: 7486
    Identification Number: https://doi.org/10.1111/1574-6968.12313
    Depositing User: Fiona Walsh
    Date Deposited: 04 Oct 2016 14:44
    Journal or Publication Title: FEMS Microbiology Letters
    Publisher: Wiley
    Refereed: Yes
    URI:

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