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    Phosphoproteomic analysis of aged skeletal muscle


    Gannon, Joan and Staunton, Lisa and O'Connell, Kathleen and Doran, Philip and Ohlendieck, Kay (2008) Phosphoproteomic analysis of aged skeletal muscle. International Journal of Molecular Medicine, 22 (1). pp. 33-42. ISSN 1107-3756

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    Abstract

    One of the most important post-translational modifications is represented by phosphorylation on tyrosine, threonine and serine residues. Since abnormal phosphorylation is associated with various pathologies, it was of interest to perform a phosphoproteomic profiling of age-related skeletal muscle degeneration. We used the fluorescent phospho-specific Pro-Q Diamond dye to determine whether changes in the overall phosphorylation of the soluble skeletal muscle proteome differs significantly between young adult and senescent fibres. As an established model system of sarcopenia, we employed 30-month-old rat gastrocnemius fibres. Following the mass spectrometric identification of 59 major 2-D phosphoprotein landmark spots, the fluorescent dye staining survey revealed that 22 muscle proteins showed a differential expression pattern between 3-month- and 30-month-old muscle. Increased phosphorylation levels were shown for myosin light chain 2, tropomyosin alpha, lactate dehydrogenase, desmin, actin, albumin and aconitase. In contrast, decreased phospho-specific dye binding was observed for cytochrome c oxidase, creatine kinase and enolase. Thus, aging-induced alterations in phosphoproteins appear to involve the contractile machinery and the cytoskeleton, as well as the cytosolic and mitochondrial metabolism. This confirms that sarcopenia of old age is a complex neuromuscular pathology that is associated with drastic changes in the abundance and structure of key muscle proteins.

    Item Type: Article
    Keywords: aging; phosphoproteome; phosphorylation; skeletal smuscle; sarcopenia; proteomics;
    Academic Unit: Faculty of Science and Engineering > Biology
    Item ID: 7500
    Identification Number: https://doi.org/10.3892/ijmm.22.1.33
    Depositing User: Prof. Kay Ohlendieck
    Date Deposited: 11 Oct 2016 15:33
    Journal or Publication Title: International Journal of Molecular Medicine
    Publisher: Spandidos Publications
    Refereed: Yes
    Funders: Science Foundation Ireland (SFI), Health Research Board (HRB)
    URI:

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