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    Illustration of Skeletal Muscle Calsequestrin Complex Formation by Cell Overlay and Two-Dimensional Blot Overlay Methodology


    Glover, Louise and Ohlendieck, Kay (2004) Illustration of Skeletal Muscle Calsequestrin Complex Formation by Cell Overlay and Two-Dimensional Blot Overlay Methodology. Basic and Applied Myology, 14 (4). pp. 231-241. ISSN 1120-9992

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    Abstract

    Based on the recent finding that a peroxidase-conjugated calsequestrin probe retains its biological binding properties, we have used it in two-dimensional blot and cell overlay procedures. Labeling of nitrocellulose replicas of electrophoretically separated microsomal proteins from predominantly fast versus slow fibres revealed self-aggregation of fast calsequestrin with molecular species of differing isolectric points. Incubation of transverse cryosections showed restricted calsequestrin interactions with elements in the fibre interior, mostly representing calsequestrin itself. This confirms that fast calsequestrin exists in large aggregates under native conditions and demonstrates the usefulness of blot and cell overlay techniques in identifying and locating supramolecular protein assemblies.

    Item Type: Article
    Keywords: blot overlay assay; calsequestrin; cell overlay assay; ryanodine receptor; triadin;
    Academic Unit: Faculty of Science and Engineering > Biology
    Item ID: 7509
    Depositing User: Prof. Kay Ohlendieck
    Date Deposited: 14 Oct 2016 10:23
    Journal or Publication Title: Basic and Applied Myology
    Refereed: Yes
    Funders: Health Research Board (HRB), European Commission
    URI:

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