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    Characterization of endospore-forming bacteria associated with entomopathogenic nematodes, Heterorhabditis spp., and description of Paenibacillus nematophilus sp. nov.


    Enright, Michael R. and McInerney, James O. and Griffin, Christine (2003) Characterization of endospore-forming bacteria associated with entomopathogenic nematodes, Heterorhabditis spp., and description of Paenibacillus nematophilus sp. nov. International Journal of Systematic and Evolutionary Microbiology, 53. pp. 435-441. ISSN 1466-5026

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    Abstract

    Endospore-forming bacteria were isolated from insect-pathogenic nematodes, Heterorhabditis spp., from three diverse geographical locations. Spindle-shaped sporangia of these bacteria adhere to the free-living infective stage of the nematode, which carries them to new insect hosts, where the bacterium reproduces. These isolates were characterized based on phenotypic and chemotaxonomic properties and 16S rRNA gene sequences. Analysis of the 16S rRNA gene placed the isolates within the genus Paenibacillus. The isolates shared higher sequence similarities with each other (95.1-100%) than they did with any other named species within the genus (89.2-94%). Paenibacillus macquariensis, Paenibacillus azoreducens, Paenibacillus amylolyticus and Paenibacillus durus were among the species with highest sequence similarity to these isolates. The isolates shared a high degree of phenotypic similarity and were easily distinguished from closely related members of the genus. Anteiso-C15:0 and C16:0 were among the major fatty acid types and the DNA G + C content was approximately 44 mol% in all isolates. DNA-DNA similarity studies revealed genomic heterogeneity among the isolates, such that they are likely to represent more than one species. Two of the isolates (both from a Heterorhabditis megidis isolate from Estonia) are phenotypically distinguishable from the others and are proposed as a single species, Paenibacillus nematophilus sp. nov. The type strain for this novel species is NEM1aT (=DSM 13559T =NCIMB 13845T). The other isolates, although closely related to the proposed species, are likely to represent at least one, but most likely two, novel species.

    Item Type: Article
    Keywords: endospore-forming bacteria; entomopathogenic nematodes; Heterorhabditis spp.; Paenibacillus nematophilus sp. nov.;
    Academic Unit: Faculty of Science and Engineering > Biology
    Item ID: 7526
    Identification Number: https://doi.org/10.1099/ijs.0.02344-0
    Depositing User: Dr. Christine Griffin
    Date Deposited: 18 Oct 2016 14:58
    Journal or Publication Title: International Journal of Systematic and Evolutionary Microbiology
    Publisher: Microbiology Society
    Refereed: Yes
    URI:

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