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    Use of Galleria mellonella larvae to evaluate the in vivo anti-fungal activity of [Ag2(mal)(phen)3]


    Rowan, Raymond and Moran, Ciara and McCann, Malachy and Kavanagh, Kevin (2009) Use of Galleria mellonella larvae to evaluate the in vivo anti-fungal activity of [Ag2(mal)(phen)3]. Biometals, 22 (3). pp. 461-467. ISSN 0966-0844

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    Abstract

    Larvae of the insect Galleria mellonella were employed to assess the in vivo antifungal efficacy of ([Ag2(mal)(phen)3]), AgNO3 and 1,10- phenanthroline. Larvae pre-inoculated with these compounds were protected from a subsequent lethal infection by the yeast Candida albicans while larvae inoculated 1 and 4 h post-infection showed significantly increased survival (P\0.01) compared to control larvae. Administration of these compounds resulted in an increase over 48 h in the density of insect haemocytes (immune cells) but there was no widespread activation of genes for antimicrobial peptides. This work demonstrates that G. mellonella larvae may be employed to ascertain the antifungal efficacy of silver(I) compounds and offers a rapid and effective means of assessing the in vivo activity of inorganic antimicrobial compounds.

    Item Type: Article
    Keywords: Antifungal; Candida albicans; Silver(I); Insect; Galleria;
    Academic Unit: Faculty of Science and Engineering > Biology
    Item ID: 7567
    Identification Number: https://doi.org/10.1007/s10534-008-9182-3
    Depositing User: Dr. Kevin Kavanagh
    Date Deposited: 24 Oct 2016 16:52
    Journal or Publication Title: Biometals
    Publisher: Springer Verlag
    Refereed: Yes
    Funders: Higher Education Authority (HEA)
    URI:

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