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    Physical stress primes the immune response of Galleria mellonella larvae to infection by Candida albicans


    Mowlds, Peter and Barron, Aoife and Kavanagh, Kevin (2008) Physical stress primes the immune response of Galleria mellonella larvae to infection by Candida albicans. Microbes and Infection, 10 (6). pp. 628-634. ISSN 1286-4579

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    Abstract

    Larvae of the greater wax moth (Galleria mellonella) that had been subjected to physical stress by shaking in cupped hands for 2 min showed reduced susceptibility to infection by Candida albicans when infected 24 h after the stress event. Physically stressed larvae demonstrated an increase in haemocyte density and elevated mRNA levels of galiomicin and an inducible metalloproteinase inhibitor (IMPI ) but not transferrin or gallerimycin. In contrast, previous work has demonstrated that microbial priming of larvae resulted in the induction of all four genes. Examination of the expression of proteins in the insect haemolymph using 2D electrophoresis and MALDI TOF analysis revealed an increase in the intensity of a number of peptides showing some similarities with proteins associated with the insect immune response to infection. This study demonstrates that non-lethal physical stress primes the immune response of G. mellonella and this is mediated by elevated haemocyte numbers, increased mRNA levels of genes coding for two antimicrobial peptides and the appearance of novel peptides in the haemolymph. This work demonstrates that physical priming increases the insect immune response but the mechanism of this priming is different to that induced by low level exposure to microbial pathogens.

    Item Type: Article
    Keywords: Galleria mellonella; Antimicrobial proteins; Candida; Host defence; 2D gel electrophoresis; MALDI TOF analysis; Gene expression;
    Academic Unit: Faculty of Science and Engineering > Biology
    Item ID: 7568
    Identification Number: https://doi.org/10.1016/j.micinf.2008.02.011
    Depositing User: Dr. Kevin Kavanagh
    Date Deposited: 24 Oct 2016 16:52
    Journal or Publication Title: Microbes and Infection
    Publisher: Elsevier
    Refereed: Yes
    Funders: Higher Education Authority (HEA)
    URI:

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