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    Facilitating co-existence by tracking gene dispersal in conventional potato systems with microsatellite markers


    Petti, Carloalberto and Meade, Conor and Downes, Martin and Mullins, Ewen (2007) Facilitating co-existence by tracking gene dispersal in conventional potato systems with microsatellite markers. Environmental Biosafety Research, 6 (4). pp. 223-235. ISSN 1635-7922

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    Abstract

    Based on international findings, Irish co-existence guidelines for the cultivation of GM potato stipulate that an isolation distance of 20 m is required to minimize the spread of transgenic pollen in accordance with required labeling thresholds. As potato tolerant to Phytophthora infestans is the most applicable GM crop from an Irish context, we tested the efficacy of this isolation distance under Irish environmental conditions using the conventional variety Désirée as a pollen donor and the male-sterile variety British Queen as a pollen receptor. Gene flow was determined by scoring for berry presence on receptor plants and confirmed using a microsatellite marker system designed to assess paternity in F(1) seedlings. 99.1% of seedlings recovered were identified as having Désirée paternity. Whereas 19.9% (140/708) of total berries formed on receptor plants occurred at a distance of 21 m from the pollen source, only 4 of these berries bore viable true potato seed (TPS), from which 23 TPS germinated. TPS-bearing berry formation was negatively correlated with distance from the pollen source, and although overall distribution of berries and seeds was non-random across the plot, no significant correlation was evident with respect to wind direction. Microsatellite markers were also used to confirm that the foraging beetle Meligethes aeneus is a vector for the transmission of potato pollen, but a more detailed statistical analysis of this dataset was limited by inclement weather during the trial. To conclude, we recommend that a two-tiered system be established in regard to establishing isolation distances for the experimental trial and commercial cultivation of GM potato in Ireland, and that responsible crop management be adopted to minimize the establishment of TPS-derived volunteers, which we have noted will emerge through a rotation as a result of pollen-mediated gene flow.

    Item Type: Article
    Keywords: Solanum tuberosum; potato; microsatellite; gene flow; co-existence;
    Academic Unit: Faculty of Science and Engineering > Biology
    Item ID: 7607
    Identification Number: https://doi.org/10.1051/ebr:2007033
    Depositing User: Dr. Conor Meade
    Date Deposited: 10 Nov 2016 15:26
    Journal or Publication Title: Environmental Biosafety Research
    Publisher: EDP Sciences
    Refereed: Yes
    URI:

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