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    Activation-Induced Expression of CD56 by T Cells Is Associated With a Reprogramming of Cytolytic Activity and Cytokine Secretion Profile In Vitro


    Kelly-Rogers, Jane and Madrigal-Estebas, Laura and O'Connor, Tony and Doherty, Derek G. (2006) Activation-Induced Expression of CD56 by T Cells Is Associated With a Reprogramming of Cytolytic Activity and Cytokine Secretion Profile In Vitro. Human Immunology, 67. pp. 863-873.

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    Abstract

    A subset of human T lymphocytes expresses the natural killer (NK) cell-associated receptor CD56 and is capable of major histocompatibility complex (MHC)-unrestricted cytotoxicity against a variety of autologous and allogeneic tumor cells. CD56+T cells have shown potential for immunotherapy as antitumor cytotoxic effectors, but their capacity to control adaptive immune responses via cytokine secretion is unclear. We have examined the inducibility of CD56+T cells from human blood in vitro and compared the kinetics of Th1, Th2, and regulatory cytokine secretion by CD56+T cells with those of conventional CD56¯ T cells. CD56 was induced on CD8+ and CD4¯CD8¯ T cells by CD3/T-cell receptor (TCR)- mediated activation, particularly when grown in the presence of interleukin (IL)-2. Activation induced CD56+ T cells proliferated less vigorously but displayed enhanced natural cytotoxicity compared with CD56¯ T cells. CD56+ T cells released interferon-y )IFN-y) and interleukin-13(IL-13), but not IL-10, upon TCR stimulation. Flow cytometric analysis demonstrated that, compared with CD56¯ T cells, elevated proportions of CD56+ T cells expressed IFN-y, IL-4, and IL-13 within hours of activation. These acquired cytolytic and cytokine secretion activities of CD56+ T cells make them potential targets for immunotherapy for infectious and immune-mediated disease.

    Item Type: Article
    Keywords: CD56+T cells: natural killer cells: cytokines: cytotoxicity: kinetics:
    Academic Unit: Faculty of Science and Engineering > Biology
    Item ID: 896
    Depositing User: Dr. Derek Doherty
    Date Deposited: 13 Feb 2008
    Journal or Publication Title: Human Immunology
    Publisher: Elsevier
    Refereed: Yes
    URI:

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