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    Modification of reactive oxygen species scavenging capacity of chloroplasts through plastid transformation.


    Poage, Miranda and Le Martret, Benedicte and Jansen, Marcel A.K. and Nugent, Gregory D. and Dix, Philip (2011) Modification of reactive oxygen species scavenging capacity of chloroplasts through plastid transformation. Plant Molecular Biology, 76. pp. 371-384.

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    Abstract

    Reactive oxygen species (ROS), including superoxide anions, hydrogen peroxide and hydroxyl radicals are generated through normal biochemical processes, but their production is increased by abiotic stresses. The prospects for enhancing ROS scavenging, and hence stress tolerance, by direct gene expression in a vulnerable cell compartment, the chloroplast, have been explored in tobacco. Several plastid transformants were generated which contained either a Nicotiana mitochondrial superoxide dismutase (MnSOD) or an Escherichia coli glutathione reductase (gor) gene. MnSOD lines had a three-fold increase in MnSOD activity, but interestingly a five to nine-fold increase in total chloroplast SOD activity. Gor transgenic lines had up to 6 times higher GR activity and up to 8 times total glutathione levels compared to wild type tobacco. Photosynthetic capacity of transplastomic plants, as measured by chlorophyll content and variable fluorescence of PSII was equivalent to non-transformed plants. The response of these transplastomic lines to several applied stresses was examined. In a number of cases improved stress tolerance was observed. Examples include enhanced methyl viologen (Paraquat)-induced oxidative stress tolerance in Mn-superoxidase dismutase overexpressing plants, improved heavy metal tolerance in glutathione reductase expressing lines, and improved tolerance to UV-B radiation in both sets of plants.

    Item Type: Article
    Keywords: Chloroplast transformation; Superoxide dismutase; Glutathione reductase; Abiotic stress;
    Academic Unit: Faculty of Science and Engineering > Biology
    Item ID: 9722
    Identification Number: https://doi.org/10.1007/s11103-011-9784-y
    Depositing User: Prof. Philip J. Dix
    Date Deposited: 26 Jul 2018 16:00
    Journal or Publication Title: Plant Molecular Biology
    Publisher: Springer
    Refereed: Yes
    URI:

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