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    Selection of hydroxyproline-resistant proline-accumulating mutants of cauliflower. C.R. Deane, M .P. Fuller' & P.J. Dix


    Deane, C.R. and Fuller, M.P. and Dix, Philip (1995) Selection of hydroxyproline-resistant proline-accumulating mutants of cauliflower. C.R. Deane, M .P. Fuller' & P.J. Dix. Euphytica, 85. pp. 329-334.

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    Official URL: https://link.springer.com/journal/10681


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    Abstract

    A procedure is described by which hydroxyproline-resistant lines could be selected from regenerating curd tissue of cauliflower. Mutagenesis was by N-nitroso-N-ethylurea, supplied as a drop of 0 .3 mM solution on each 3 mm diameter curd piece. The mutagen generated numerous morphological and pigment mutations without significantly affecting shoot regeneration from explants . Thirty one resistant shoots were recovered from more than six thousand explants mutagenised on regeneration medium supplemented with 3 mM hydroxyproline, while none was obtained from a similar number of non-mutagenised controls . Out of twenty-three resistant shoots which survived subculture, only one showed consistently elevated levels of endogenous proline . During early shoot culture passages, proline levels were 3 .6-4.7 times higher than controls, but this was reduced to 1 .6 times after 10-12 culture passages in the absence of hydroxyproline . Possible reasons for this decline are discussed . Leaf strip assays suggest resistant shoots may be chimeras and current efforts are directed towards regenerating solid mutants from resistant sectors . These will then be evaluated for any alteration in frost tolerance .

    Item Type: Article
    Keywords: curd culture; frost tolerance; N-nitroso-N-ethylurea; proline accumulation; mutagenesis;
    Academic Unit: Faculty of Science and Engineering > Biology
    Item ID: 9728
    Depositing User: Prof. Philip J. Dix
    Date Deposited: 27 Jul 2018 13:02
    Journal or Publication Title: Euphytica
    Publisher: luwer Academic Publishers
    Refereed: Yes
    URI:

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