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    Impact of the stringency of cell selection on plastid segregation in protoplast fusion-derived Nicotiana regenerates.


    Malone, R. and Horvfith, G.V. and Cseplo, A. and Bfizfis, B. and Dix, Philip and Medgyesy, P. (1992) Impact of the stringency of cell selection on plastid segregation in protoplast fusion-derived Nicotiana regenerates. Theoretical and Applied Genetics, 84. pp. 866-873.

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    Official URL: https://link.springer.com/journal/122


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    Abstract

    Vegetative segregation of a mixed plastid population in protoplast fusion-derived cell lines can be directed by a selection favouring the multiplication of one of the parental plastid types. This report defines some of the critical conditions leading to a homogeneous plastid population in cybrid plants generated by protoplast fusion between Nicotiana plumbaginifolia and an albino and streptomycin-resistant N. tabacum plastid mutant. Light (1,500 Ix) conferred a strong selective advantage to chloroplasts versus albino plastids, while the lack of this effect in dim light (300 Ix) indicated that a sufficient light intensity is essential to the phenomenon. Selection on streptomycin-containing medium in the dark, however, led to the preferential multiplication of resistant plastids. Streptomycin selection of resistant chloroplasts in the light, consequently, results in a plastid selection of doubled stringency. In another experiment a definite, but leaky, selection for chloroplast recombination (selection for greening on streptomycin-containing medium in dim light) was used to reveal various recombination products. Protoplast fusion in fact resulted in cybrid plants showing only simple chimeric segregation of unchanged parental plastids. These results demonstrate the essential requirement for stringent plastid selection, as defined by cell culture conditions, to precede the formation of shoots expected to possess the desired plastid genetic composition.

    Item Type: Article
    Keywords: Nicotiana; Plastid genetic markers; Plastid segregation; Plastid selection; Protoplast fusion;
    Academic Unit: Faculty of Science and Engineering > Biology
    Item ID: 9729
    Depositing User: Prof. Philip J. Dix
    Date Deposited: 27 Jul 2018 13:43
    Journal or Publication Title: Theoretical and Applied Genetics
    Publisher: Springer
    Refereed: Yes
    URI:

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