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    Mutagenesis and Triazine Herbicide Effects in Strawberry Shoot Cultures.


    Malone, R.P. and Dix, Philip (1990) Mutagenesis and Triazine Herbicide Effects in Strawberry Shoot Cultures. Journal of Expermental Botany, 41 (225). pp. 463-469.

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    Abstract

    The effects of simazine on shoot culture of two varieties of strawberry were investigated with a view to establishing a system for in vitro selection of resistant mutants. In single shoots some chlorophyll loss occurred at herbicide levels producing little growth inhibition, but efficient bleaching was associated with severe suppression of growth. Varying simazine and sucrose levels, in both single and multiple shoot cultures did not reveal any conditions where chlorophyll destruction and growth inhibition could be separated. A protective effect, against bleaching, of high sucrose levels was not linked to suppression of photosynthesis, as assessed by CO2-fixation measurements. Mutagenesis with nitrosomethyl urea resulted in chlorophyll deficiencies in about 10% of shoots arising from axillary buds, but no resistant sectors emerged from 8000 and 400 mutagenized explants placed on medium containing inhibitory levels of simazine and streptomycin sulphate, respectively.

    Item Type: Article
    Keywords: Strawberry; simazine; triazine; mutagenesis; shoot cultures;
    Academic Unit: Faculty of Science and Engineering > Biology
    Item ID: 9730
    Depositing User: Prof. Philip J. Dix
    Date Deposited: 27 Jul 2018 14:00
    Journal or Publication Title: Journal of Expermental Botany
    Refereed: Yes
    URI:

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