Ao, Xiang and Bright, Sandra A. and Taylor, N.C. and Elmes, Robert B.P.
(2017)
2-Nitroimidazole based fluorescent probes
for nitroreductase; monitoring reductive stress
in cellulo.
Organic & Biomolecular Chemistry, 15.
pp. 6104-6108.
ISSN 1477-0520
Abstract
Two 2-nitroimidazole-1,8-naphthalimide conjugates, 1 and 2, have been synthesised as fluorescence probes for the detection of reductive stress in HeLa cells. The 4-substituted derivative 1 was shown to act as a highly sensitive and selective substrate for nitroreductase where it exhibited a clear blue to green ratiometric fluorescence response visible to the naked eye. Moreover, biological studies demonstrated 1 could be activated in cellulo where the impact of reductive stress was easily monitored using confocal microscopy and flow cytommetry.
| Item Type: |
Article
|
| Additional Information: |
Cite as: Org. Biomol. Chem., 2017,
15, 6104 |
| Keywords: |
2-Nitroimidazole; fluorescent probes; nitroreductase; monitoring reductive stress; HeLa cells; |
| Academic Unit: |
Faculty of Science and Engineering > Chemistry |
| Item ID: |
11631 |
| Identification Number: |
https://doi.org/10.1039/C7OB01406F |
| Depositing User: |
Robert Elmes
|
| Date Deposited: |
05 Nov 2019 15:25 |
| Journal or Publication Title: |
Organic & Biomolecular Chemistry |
| Publisher: |
Royal Society of Chemistry |
| Refereed: |
Yes |
| URI: |
|
| Use Licence: |
This item is available under a Creative Commons Attribution Non Commercial Share Alike Licence (CC BY-NC-SA). Details of this licence are available
here |
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