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    'AND'-based fluorescence scaffold for the detection of ROS/RNS and a second analyte


    Odyniec, Maria L. and Sedgwick, Adam C. and Swan, Alexander H. and Weber, Maria and Tang, T.M. Simon and Gardiner, Jordan E. and Zhang, Miao and Jiang, Yun-Bao and Kociok-Kohn, Gabriele and Elmes, Robert B.P. and Bull, Steven D. and He, Xiao-Peng and James, Tony D. (2018) 'AND'-based fluorescence scaffold for the detection of ROS/RNS and a second analyte. Chemical Communications, 54 (61). pp. 8466-8469. ISSN 1359-7345

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    Abstract

    Traditionally, fluorescence probes have focused on the detection of a single biomarker for a specific process. In this work, we set out to develop a number of fluorescence probes that enable the detection of a chosen analyte in the presence of reactive oxygen/nitrogen species (ROS/RNS). These fluorescence probes when activated result in the formation of the highly fluorescent pink dye, resorufin. Therefore, we have labelled these fluorescent probes as ‘Pinkments’. Our first ‘Pinkment’ was shown to detect biologically relevant concentrations of ONOO− and have an excellent selectivity against other ROS/RNS. Pinkment-OH was developed to provide a core unit which could be easily functionalised to produce a range of ‘AND’ based fluorescence probes for the detection of ROS/RNS and a second analyte. For proof of concept, we synthesised Pinkment-OTBS and Pinkment-OAc. These ‘AND’-based probes were successfully shown to detect ROS/RNS and F− or esterase, respectively.

    Item Type: Article
    Keywords: Fluorogenic Probe; Nitric-Oxide; Peroxynitrite; Gluthatione; Cells;
    Academic Unit: Faculty of Science and Engineering > Chemistry
    Item ID: 12532
    Identification Number: https://doi.org/10.1039/C8CC04316G
    Depositing User: Robert Elmes
    Date Deposited: 06 Mar 2020 16:55
    Journal or Publication Title: Chemical Communications
    Publisher: Royal Society of Chemstry
    Refereed: Yes
    URI:

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