O'Connor, John J. and O'Boyle, K.M. and Lowry, John P. (2017) Effects of a combination of 3,4-methylenedioxymeth amphetamine and caffeine on real time stimulated dopamine release in the rat striatum: studies using fast cyclic voltammetry. Journal of Neuroscience Methods, 300. pp. 216-223. ISSN 0165-0270

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Abstract

It is well documented that caffeine exacerbates the hyperthermia associated with acute exposure to 3,4-methylenedioxymethamphetamine (MDMA) in rats. Previous reports have also indicated that MDMA-related enhancement of dopamine release is exacerbated in the presence of caffeine. In the present study we have examined whether the effects of MDMA on real-time stimulated dopamine release in the absence of uptake inhibition are accentuated in the presence of caffeine. Isolated striatal slices from adult male Wistar rats were treated acutely with MDMA, caffeine, or a combination, and their effects on single and 5 pulse stimulated dopamine release monitored using the technique of fast cyclic voltammetry. Caffeine at 10 or 100μM had no significant effect on single pulse stimulated dopamine release. However 100μM caffeine caused a significant peak increase in 5 pulse stimulated dopamine release. Both 1 and 30μM MDMA gave rise to a significant increase in both single and 5-pulse dopamine release and reuptake. A combination of 100 μM caffeine and 1 or 30μM MDMA did not significantly enhance the effects of MDMA on single or 5 pulse dopamine release and reuptake when compared to that applied alone. Utilizing single action potential dependent dopamine release, these results do not demonstrate a caffeine-enhanced MDMA-induced dopamine release.

Item Type:	Article
Keywords:	Caffeine; MDMA; real time dopamine release; cyclic voltammetry; single pulse; rat striatum;
Academic Unit:	Faculty of Science and Engineering > Chemistry
Item ID:	13201
Identification Number:	https://doi.org/10.1016/j.jneumeth.2017.08.026
Depositing User:	John Lowry
Date Deposited:	28 Aug 2020 13:55
Journal or Publication Title:	Journal of Neuroscience Methods
Publisher:	Elsevier
Refereed:	Yes
URI:	Publisher
Use Licence:	This item is available under a Creative Commons Attribution Non Commercial Share Alike Licence (CC BY-NC-SA). Details of this licence are available here

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