MURAL - Maynooth University Research Archive Library



    Chemical Modification Alters Protein-Protein Interactions and Can Lead to Lower Protein Solubility


    Quinn, Michelle K and James, Susan and McManus, Jennifer J. (2019) Chemical Modification Alters Protein-Protein Interactions and Can Lead to Lower Protein Solubility. Journal of Physical Chemistry B, 123 (20). pp. 4373-4379. ISSN 1520-6106

    [img]
    Preview
    Download (913kB) | Preview


    Share your research

    Twitter Facebook LinkedIn GooglePlus Email more...



    Add this article to your Mendeley library


    Abstract

    The chemical modification of proteins is at the frontier of developments in biological imaging and biopharmaceutics. With the advent of more sensitive and higher resolution imaging techniques, researchers increasingly rely on the functionalization of proteins to enable these techniques to capture cellular processes. For biopharmaceutical therapies, chemically modified proteins, for example, antibody-drug conjugates (ADCs) offer the possibility of more tailored treatments for the disease with lower toxicities than traditional small molecule therapies. However, relatively little consideration is paid to how chemical modifications impact protein−protein interactions and solution stability. Using human γD-crystallin as a model, we demonstrate that chemical modification of the protein surface alters protein−protein interactions, which can result in lower solubility depending on the chemical nature of the modifier and the position on the protein where the modification is made. Understanding these effects is essential to ensure that modifying proteins effectively occurs with minimum self-association and that studies carried out using labeled proteins accurately reflect those of unmodified proteins.

    Item Type: Article
    Keywords: Chemical modification; alters protein; protein interactions; Lower Protein Solubility;
    Academic Unit: Faculty of Science and Engineering > Chemistry
    Item ID: 13456
    Identification Number: https://doi.org/10.1021/acs.jpcb.9b02368
    Depositing User: IR Editor
    Date Deposited: 28 Oct 2020 10:45
    Journal or Publication Title: Journal of Physical Chemistry B
    Publisher: American Chemical Society
    Refereed: Yes
    URI:
    Use Licence: This item is available under a Creative Commons Attribution Non Commercial Share Alike Licence (CC BY-NC-SA). Details of this licence are available here

    Repository Staff Only(login required)

    View Item Item control page

    Downloads

    Downloads per month over past year

    Origin of downloads