Glynn, Macdara and Kirby, Dan and Chung, Danielle and Kinahan, David J. and Kijanka, Gregor and Ducrée, Jens (2014) Centrifugo-Magnetophoretic Purification of CD4+ Cells from Whole Blood Toward Future HIV/AIDS Point-of-Care Applications. Journal of Laboratory Automation, 19 (3). pp. 285-296. ISSN 1540-2452

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Abstract

In medical diagnostics, detection of cells exhibiting specific phenotypes constitutes a paramount challenge. Detection technology must ensure efficient isolation of (often rare) targets while eliminating nontarget background cells. Technologies exist for such investigations, but many require high levels of expertise, expense, and multistep protocols. Increasing automation, miniaturization, and availability of such technologies is an aim of microfluidic lab-on-a-chip strategies. To this end, we present an integrated, dual-force cellular separation strategy using centrifugo-magnetophoresis. Whole blood spiked with target cells is incubated with (super-)paramagnetic microparticles that specifically bind phenotypic markers on target cells. Under rotation, all cells sediment into a chamber located opposite a co-rotating magnet. Unbound cells follow the radial vector, but under the additional attraction of the lateral magnetic field, bead-bound target cells are deflected to a designated reservoir. This multiforce separation is continuous and low loss. We demonstrate separation efficiently up to 92% for cells expressing the HIV/AIDS relevant epitope (CD4) from whole blood. Such highly selective separation systems may be deployed for accurate diagnostic cell isolations from biological samples such as blood. Furthermore, this high efficiency is delivered in a cheap and simple device, thus making it an attractive option for future deployment in resource-limited settings.

Item Type:	Article
Additional Information:	Funding: The authors disclosed receipt of the following financial support for the research, authorship, and/or publication of this article: This work was supported by Enterprise Ireland (grant CF 2011 1317), the ERDF, and the Science Foundation Ireland (grant 10/CE/B1821). Cite as: Glynn M, Kirby D, Chung D, Kinahan DJ, Kijanka G, Ducrée J. Centrifugo-Magnetophoretic Purification of CD4+ Cells from Whole Blood Toward Future HIV/AIDS Point-of-Care Applications. Journal of Laboratory Automation. 2014;19(3):285-296. doi:10.1177/2211068213504759
Keywords:	HIV/AIDS diagnostics; cell purification; cell separation; centrifugal microfluidics; lab-on-a-disc;
Academic Unit:	Faculty of Science and Engineering > Experimental Physics
Item ID:	13942
Identification Number:	https://doi.org/10.1177/2211068213504759
Depositing User:	Dan Nickstrom
Date Deposited:	05 Feb 2021 09:58
Journal or Publication Title:	Journal of Laboratory Automation
Publisher:	Society for Laboratory Automation and Screening/Sage Publications
Refereed:	Yes
Funders:	Enterprise Ireland, European Regional Development Fund (ERDF), Science Foundation Ireland (SFI)
URI:	Publisher Organisation
Use Licence:	This item is available under a Creative Commons Attribution Non Commercial Share Alike Licence (CC BY-NC-SA). Details of this licence are available here

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