Kirby, Dan and Bailey, Éanna and Glynn, Macdara and Nwankire, Charles and Ducrée, Jens (2014) Electromagnetic Trapping of Cancer Cells on an Array of Thin-Film Permalloy Microfeatures for Single Cell Analysis. In: The 18th International Conference on Miniaturized Systems for Chemistry and Life Sciences. Chemical and Biological Microsystems Society (CBMS). ISBN 9781634396974

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Abstract

Isolation of individual cells from a bulk sample is vital in cell based diagnostics. Previous work has established the ability to arrange target cells into ordered arrays, using physical barriers such as micropillars and cups [1]. We here present a magnetic separation device that allows the tagged cells of interest to align into a highly ordered array where they are selected based on both immuno-markers and size. But, unlike the physical barrier methods, our device is able to trap the cells using an only 160-nm thin film of permalloy (80% Nickel, 20% Iron) micropatterned on the base of the chamber (Fig. 1). Maxima of the magnetic field arise in the vicinity of the sharp edges of these localized micro-spots (Fig. 2), thereby creating potential wells to localize paramagnetic bioparticles such as tagged cells or beads during analysis or successive washing steps (Fig. 3). Compared to a complex network of physical barriers, the trapped cells can simply be released by turning off the external magnet, allowing for further downstream analysis or removal to waste.

Item Type:	Book Section
Keywords:	Electromagnetic Trapping; Array; Cancer Cells; Thin-Film Permalloy Microfeatures; Single Cell Analysis;
Academic Unit:	Faculty of Science and Engineering > Experimental Physics
Item ID:	13945
Depositing User:	Dan Nickstrom
Date Deposited:	05 Feb 2021 11:17
Publisher:	Chemical and Biological Microsystems Society (CBMS)
Refereed:	Yes
URI:
Use Licence:	This item is available under a Creative Commons Attribution Non Commercial Share Alike Licence (CC BY-NC-SA). Details of this licence are available here

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