Glynn, Macdara and Kirby, Dan and Nwankire, Charles and Spillane, Cathy and Shiels, Orla and O'Leary, John and Ducrée, Jens
(2014)
Staging the clinical status from blood of cancer patients by chip-based cell enumeration following targeted removal of normal cells.
In:
18th International Conference on Miniaturized Systems for Chemistry and Life Sciences (MicroTAS 2014).
Chemical and Biological Microsystems Society (CBMS).
ISBN 9781634396974
Abstract
Even though an agreed phenotypic definition of circulating tumor cells (CTCs) remains elusive in the literature,
many current detection technologies isolate candidate cells based on molecular recognition of cellular epitopes that
may not accurately predict CTC load. Rather than using such an epitope specific “positive-capture” strategy, we
present a chip-based, centrifugal microfluidic platform integrating “negative-capture” magnetophoretic removal of
normal white blood cells (WBCs) from a sample and subsequent, array-based enumeration of individualized,
(untagged) abnormal cells. We compared the numerical recovery of cells on the array with the status of the donor
patient, showing that the chip can has the potential to indicate the oncogenic severity of the blood donor.
| Item Type: |
Book Section
|
| Additional Information: |
Fudning: This work was supported by Enterprise Ireland under Grant No CF 2011 1317, the ERDF and the Science Foundation Ireland under grant No 10/CE/B1821. |
| Keywords: |
Lab-on-a-Disc; Magnetic Selection; Magnetophoresis; CTC detection; |
| Academic Unit: |
Faculty of Science and Engineering > Experimental Physics |
| Item ID: |
13949 |
| Depositing User: |
Dan Nickstrom
|
| Date Deposited: |
05 Feb 2021 12:28 |
| Publisher: |
Chemical and Biological Microsystems Society (CBMS) |
| Refereed: |
Yes |
| Funders: |
Enterprise Ireland, European Regional Development Fund (ERDF), Science Foundation Ireland (SFI) |
| URI: |
|
| Use Licence: |
This item is available under a Creative Commons Attribution Non Commercial Share Alike Licence (CC BY-NC-SA). Details of this licence are available
here |
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