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    Determination of prostate cancer biomarker acid phosphatase at a copper phthalocyanine-modified screen printed gold transducer


    Fredj, Zina and Ali, Mounir Ben and Abbas, Mohammed Nooredeen and Dempsey, Eithne (2019) Determination of prostate cancer biomarker acid phosphatase at a copper phthalocyanine-modified screen printed gold transducer. Analytica Chimica Acta, 1057. pp. 98-105. ISSN 0003-2670

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    Abstract

    In this work, a novel sensor based on immobilised copper phthalocyanine, 2,9,16,23-tetracarboxylic acidpolyacrylamide (Cu(II)TC Pc-PAA) was developed for determination of acid phosphatase (ACP) levels in nanomolar quantities. Detection was based on the measurement of enzymatically generated phosphate, with initial studies focused on phosphate detection at a Cu(II)TC Pc-PAA modified screen-printed gold transducer. The sensor was characterised in relation to operational performance (pH, response time, stability, linearity, and sensitivity) and common anionic interferents (nitrate, sulphate, chloride, and perchlorate). The functionalised surface also facilitated rapid detection of the enzyme bi-product 2- naphthol over the range 5e3000 mM. Quantitation of ACP was demonstrated, realising a linear response range of 0.5e20 nM and LOD of 0.5 nM, which is within the clinical range for this prostate cancer biomarker.

    Item Type: Article
    Additional Information: Funding: This work was partially funded by the “SMARTCANCERSENS” project from the European Communities Seventh Framework Program under the Marie Curie IRSES staff exchange programme - Grant Agreement PIRSES-GA 2012-318053. Cite as: Zina Fredj, Mounir Ben Ali, Mohammed Nooredeen Abbas, Eithne Dempsey, Determination of prostate cancer biomarker acid phosphatase at a copper phthalocyanine-modified screen printed gold transducer, Analytica Chimica Acta, Volume 1057, 2019, Pages 98-105, ISSN 0003-2670, https://doi.org/10.1016/j.aca.2018.12.058.
    Keywords: Copper phthalocyanine; Phosphate; Naphthol; Acid phosphatase; Gold screen-printed electrode;
    Academic Unit: Faculty of Science and Engineering > Chemistry
    Faculty of Science and Engineering > Research Institutes > Human Health Institute
    Item ID: 13974
    Identification Number: https://doi.org/10.1016/j.aca.2018.12.058
    Depositing User: Eithne Dempsey
    Date Deposited: 08 Feb 2021 17:14
    Journal or Publication Title: Analytica Chimica Acta
    Publisher: Elsevier
    Refereed: Yes
    Funders: European Communities Seventh Framework Program
    URI:

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