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    Impedimetric and electrochemical evaluation of a new redox active steroid derivative for hormone immunosensing


    Kelch, Jessica and Delaney, Aoife and Kelleher, Fintan and Baker, Priscilla and Iwuoha, Emmanuel and Dempsey, Eithne (2020) Impedimetric and electrochemical evaluation of a new redox active steroid derivative for hormone immunosensing. Biosensors and Bioelectronics, 150 (111876). ISSN 0956-5663

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    Abstract

    Preparation and electrochemical interrogation of a novel redox active progesterone derivative progesterone thiosemicarbazone (PATC) is presented here together with an investigation into its suitability as conjugate in progesterone hormone immunosensing. PATC synthesis involved a condensation reaction between progesterone acetate and thiosemicarbazone hydrochloride. Voltammetric and pulse techniques confirmed the redox behaviour of the new compound with concentration and scan rate dependant irreversible behaviour evident at glassy carbon and gold transducers - ko (standard heterogeneous rate constant) was 2.56 � 10-3 cm2 /s (ν ¼ 100 mV/s in non-aqeuous media). Bioaffinity studies towards anti-progesterone antibodies involved a competitive ELISA format (optical) which confirmed recognition of the new progesterone derivative. Electrochemical impedance spectroscopy was employed as an interrogation technique in order to establish optimum binding and surface conditions for progesterone antigen-antibody interaction with the assistance of a redox probe (potassium hexacyanoferrate).

    Item Type: Article
    Additional Information: Funding: The authors would like to acknowledge funding under the Science Foundation Ireland Investigator Programme 12/IP/1275, and mobility funding under Marie Curie Actions – International Research Staff Exchange GA318053. Cite as: Jessica Kelch, Aoife Delaney, Fintan Kelleher, Priscilla Baker, Emmanuel Iwuoha, Eithne Dempsey, Impedimetric and electrochemical evaluation of a new redox active steroid derivative for hormone immunosensing, Biosensors and Bioelectronics, Volume 150, 2020, 111876, ISSN 0956-5663, https://doi.org/10.1016/j.bios.2019.111876.
    Keywords: Electrochemical; impedance; spectroscopy; Progesterone; thiosemicarbazone; Progesterone; immunoassay;
    Academic Unit: Faculty of Science and Engineering > Research Institutes > Human Health Institute
    Item ID: 13975
    Identification Number: https://doi.org/10.1016/j.bios.2019.111876
    Depositing User: Eithne Dempsey
    Date Deposited: 08 Feb 2021 17:19
    Journal or Publication Title: Biosensors and Bioelectronics
    Publisher: Elsevier
    Refereed: Yes
    Funders: Science Foundation Ireland Investigator Programme
    URI:

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