Grundzi, Ales
(2021)
Squaramide-Naphthalimide Conjugates as
Potential Self-Assembled Materials and as
DNA Binders.
Masters thesis, National University of Ireland Maynooth.
Abstract
This thesis entitled ‘Squaramide-Naphthalimide Conjugates as Potential Self-Assembled
Materials and as DNA Binders’ is divided in 7 chapters. Chapter 1 presents an introduction to
DNA, its function and structure. Various DNA binders were introduced in this section,
specifically currently studied naphthalimides as DNA intercalators, with potential as possible
treatments for various types of cancers. The H-bonding ability of squaramide and its potential
as a DNA binder to the negatively charged sugar phosphate backbone was discussed. Bis-intercalators were also described in detail and their strong DNA bis-intercalating ability was
also discussed. The proposed target bis-intercalators were introduced, with multiple modes of
binding to DNA, where the naphthalimide moieties would bis-intercalate into DNA, while the
squaramide would bind to the negatively charged sugar phosphate backbone of DNA.
In Chapter 2, the design and synthesis of novel compounds are discussed. The syntheses of the
desired compounds, the challenges encountered during synthesis and the change in the design
of the desired compounds was also described. Each compound was characterised with room
temperature NMR and it was concluded that after VT-NMR studies were performed, which
clearly indicated self-assembly properties of these novel compounds.
Chapter 3 describes the self-assembly properties of the novel squaramide containing bis-naphthalimides, which will involve various techniques including VT-NMR, UV absorbance
time studies, extinction coefficient studies and Scanning Electron Microscopy.
Chapter 4 discusses the possible DNA binding ability of the novel synthesised compounds
using UV-Vis absorbance, fluorescence and Ethidium Bromide Assays.
Chapter 5 gives an overall conclusion of the entire work carried out in this research and also
provides a perspective with the ideas for future work.
Chapter 6 contains the general experimental procedures including the synthesis and detailed
characterisation of each of the synthesised compounds.
Chapter 7, the Appendix section contains all supporting information.
| Item Type: |
Thesis
(Masters)
|
| Keywords: |
Squaramide-Naphthalimide Conjugates; Potential Self-Assembled Materials; DNA Binders; |
| Academic Unit: |
Faculty of Science and Engineering > Chemistry |
| Item ID: |
14949 |
| Depositing User: |
IR eTheses
|
| Date Deposited: |
21 Oct 2021 11:25 |
| URI: |
|
| Use Licence: |
This item is available under a Creative Commons Attribution Non Commercial Share Alike Licence (CC BY-NC-SA). Details of this licence are available
here |
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