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    Glycosylation in Indolent, Significant and Aggressive Prostate Cancer by Automated High-Throughput N-Glycan Profiling


    Gilgunn, Sarah and Murphy, Keefe and Stöckmann, Henning and Conroy, Paul J. and Murphy, T. Brendan and Watson, R. William and O’Kennedy, Richard J. and Rudd, Pauline M. and Saldova, Radka (2020) Glycosylation in Indolent, Significant and Aggressive Prostate Cancer by Automated High-Throughput N-Glycan Profiling. International Journal of Molecular Sciences, 21 (9233). ISSN 1422-0067

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    Abstract

    The diagnosis and treatment of prostate cancer (PCa) is a major health-care concern worldwide. This cancer can manifest itself in many distinct forms and the transition from clinically indolent PCa to the more invasive aggressive form remains poorly understood. It is now universally accepted that glycan expression patterns change with the cellular modifications that accompany the onset of tumorigenesis. The aim of this study was to investigate if differential glycosylation patterns could distinguish between indolent, significant, and aggressive PCa. Whole serum N-glycan profiling was carried out on 117 prostate cancer patients’ serum using our automated, high-throughput analysis platform for glycan-profiling which utilizes ultra-performance liquid chromatography (UPLC) to obtain high resolution separation of N-linked glycans released from the serum glycoproteins. We observed increases in hybrid, oligomannose, and biantennary digalactosylated monosialylated glycans (M5A1G1S1, M8, and A2G2S1), bisecting glycans (A2B, A2(6)BG1) and monoantennary glycans (A1), and decreases in triantennary trigalactosylated trisialylated glycans with and without core fucose (A3G3S3 and FA3G3S3) with PCa progression from indolent through significant and aggressive disease. These changes give us an insight into the disease pathogenesis and identify potential biomarkers for monitoring the PCa progression, however these need further confirmation studies.

    Item Type: Article
    Additional Information: © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/) Cite as: Gilgunn, S.; Murphy, K.; Stöckmann, H.; Conroy, P.J.; Murphy, T.B.; Watson, R.W.; O’Kennedy, R.J.; Rudd, P.M.; Saldova, R. Glycosylation in Indolent, Significant and Aggressive Prostate Cancer by Automated High-Throughput N-Glycan Profiling. Int. J. Mol. Sci. 2020, 21, 9233. https://doi.org/10.3390/ijms21239233
    Keywords: prostate cancer; biomarkers; glycosylation; indolent; aggressive; significant;
    Academic Unit: Faculty of Science and Engineering > Mathematics and Statistics
    Item ID: 15556
    Identification Number: https://doi.org/10.3390/ijms21239233
    Depositing User: Keefe Murphy
    Date Deposited: 22 Feb 2022 17:00
    Journal or Publication Title: International Journal of Molecular Sciences
    Publisher: MDPI
    Refereed: Yes
    URI:

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