Dempsey, Eithne and Rathod, Dhanraj (2018) Disposable Printed Lateral Flow Electrochemical Immunosensors for Human Cardiac Troponin T. IEEE Sensors Journal, 18 (5). pp. 1828-1834. ISSN 1530-437X

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Abstract

Here we report an electrochemical ELISA approach for cardiac Troponin T (cTnT) determination based on a lateral flow membrane with underlying screen printed electrodes ( $<100~\Omega $ /cm2). The thick film transducer was modified with the anti-cardiac Troponin T antibody via physisorption and the electrochemical performance of the immunosensor was evaluated using cyclic voltammetry. The capture antibody coated immunoelectrode employed for electrochemical determination of Troponin T antigen used a sandwich assay format with horseradish peroxidase conjugated signaling antibodies held in the track of a lateral flow sensor strip. A simple two step procedure realized signal acquisition within <20 min (total assay time). The lateral flow electrochemical immunosensor response resulted in a calibration curve with linear response (0–700 ng/ml cTnT) with limit of detection of 0.15 ng/ml.

Item Type:	Article
Keywords:	Immunosensor; Troponin T; lateral flow immunoassay;
Academic Unit:	Faculty of Science and Engineering > Chemistry
Item ID:	16102
Identification Number:	https://doi.org/10.1109/JSEN.2018.2789436
Depositing User:	Eithne Dempsey
Date Deposited:	14 Jun 2022 15:22
Journal or Publication Title:	IEEE Sensors Journal
Publisher:	Institute of Electrical and Electronics Engineers
Refereed:	Yes
URI:	Publisher
Use Licence:	This item is available under a Creative Commons Attribution Non Commercial Share Alike Licence (CC BY-NC-SA). Details of this licence are available here

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