García‐Béjar, Beatriz and Owens, Rebecca A. and Briones, Ana and Arévalo‐Villena, María
(2021)
Proteomic profiling and glycomic analysis of the yeast cell wall in strains with Aflatoxin B 1 elimination ability.
Environmental Microbiology, 23 (9).
pp. 5305-5319.
ISSN 1462-2912
Abstract
The use of microorganisms for Aflatoxin B1 elimination has been studied as a new alternative tool and it
is known that cell wall carried out a critical role. For
that reason, cell wall and soluble intracellular fraction
of eight yeasts with AFB1 detoxification capability
were analysed. The quantitative and qualitative comparative label-free proteomic allowed the identification of diverse common constituent proteins, which
revealed that putative cell wall proteins entailed less
than 10% of the total proteome. It was possible to
characterize different enzymes linked to cell wall
polysaccharides biosynthesis as well as other proteins related with the cell wall organization and regulation. Additionally, the concentration of the principal
polysaccharides was determined which permitted us
to observe that β-glucans concentration was higher
than mannans in most of the samples. In order to better understand the biosorption role of the cell wall
against the AFB1, an antimycotic (Caspofungin) was
used to damage the cell wall structure. This assay
allowed the observation of an effect on the normal
growth of those yeasts with damaged cell walls that
were exposed to AFB1. This effect was not observed
in yeast with intact cell walls, which may reveal a pro�tective role of this structure against mycotoxins
| Item Type: |
Article
|
| Additional Information: |
Cite as:García-Béjar, B., Owens, R.A., Briones, A. and Arévalo-Villena, M. (2021), Proteomic profiling and glycomic analysis of the yeast cell wall in strains with Aflatoxin B1 elimination ability. Environ Microbiol, 23: 5305-5319. https://doi.org/10.1111/1462-2920.15606 |
| Keywords: |
Aflatoxin B; Proteomic analysis; |
| Academic Unit: |
Faculty of Science and Engineering > Research Institutes > Human Health Institute |
| Item ID: |
17343 |
| Identification Number: |
https://doi.org/10.1111/1462-2920.15606 |
| Depositing User: |
Rebecca Owens
|
| Date Deposited: |
22 Jun 2023 11:36 |
| Journal or Publication Title: |
Environmental Microbiology |
| Publisher: |
Wiley |
| Refereed: |
Yes |
| URI: |
|
| Use Licence: |
This item is available under a Creative Commons Attribution Non Commercial Share Alike Licence (CC BY-NC-SA). Details of this licence are available
here |
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