Soon, Geok Hong and Deasy, Mary and Dempsey, Eithne (2021) An Electrochemical Evaluation of Novel Ferrocene Derivatives for Glutamate and Liver Biomarker Biosensing. Biosensors, 11 (8). p. 254. ISSN 2079-6374

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Abstract

Here, we present an evaluation of two new monosubstituted ferrocene (Fc) derivatives, 3- (1H-pyrrol-1-yl)propanamidoferrocene and 1-hydroxy-2-[2-(thiophen-3-yl)-ethylamino]ethylferrocene, as glutamate oxidase mediators, together with their preparation and characterisation. Taking into consideration the influence of the electronic effects of substituents on the redox potentials of the Fc species, two candidates with pyrrole or thiophene moieties were proposed for investigation. Film studies involved potential sweeping in the presence of pyrrole or 3,4-ethylenedioxythiophene monomers resulting in stable electroactive films with % signal loss upon cycling ranging from 1 to 7.82% and surface coverage (Γ) 0.47–1.15 × 10−9 mol/cm2 for films formed under optimal conditions. Construction of a glutamate oxidase modified electrode resulted in second-generation biosensing with the aid of both cyclic voltammetry and hydrodynamic amperometry, resulting in glutamate sensitivity of 0.86–1.28 µA/mM and Km (app) values over the range 3.67–5.01 mM. A follow-up enzyme assay for liver biomarker γ-glutamyl transpeptidase realised unmediated and mediated measurement establishing reaction and incubation time investigations and a realising response over <100 U/L γ-glutamyl transpeptidase with a sensitivity of 5 nA/UL−1

Item Type:	Article
Additional Information:	Cite as: Soon, G.H.; Deasy, M.; Dempsey, E. An Electrochemical Evaluation of Novel Ferrocene Derivatives for Glutamate and Liver Biomarker Biosensing. Biosensors 2021, 11, 254. https://doi.org/10.3390/bios11080254
Keywords:	glutamate biosensor; liver biomarker; γ-glutamyl transpeptidase; electrochemical sensing; ferrocenes; polymer
Academic Unit:	Faculty of Science and Engineering > Chemistry Faculty of Science and Engineering > Research Institutes > Human Health Institute
Item ID:	17380
Identification Number:	https://doi.org/10.3390/bios11080254
Depositing User:	Eithne Dempsey
Date Deposited:	06 Jul 2023 09:41
Journal or Publication Title:	Biosensors
Publisher:	MDPI
Refereed:	Yes
URI:	Publisher
Use Licence:	This item is available under a Creative Commons Attribution Non Commercial Share Alike Licence (CC BY-NC-SA). Details of this licence are available here

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