Halpin, Grace and Herdman, Karen and Dempsey, Eithne (2021) Electrochemical investigations into enzymatic polymerisation of 1,10-phenanthroline-5,6-dione as a redox mediator for lactate sensing. Sensors and Actuators Reports, 3. p. 100032. ISSN 2666-0539
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Abstract
Lactate biosensor fabrication using a chitosan and cross linker configuration realised solution mediation(K3Fe (CN)6) with lactate linear range9.9 × 10−4 to 5.66×10−3 M, sensitivity 1.44×10−3C cm−2 mM−1 and LOD of 0.54 mM. Further development involved use of the heterocyclic quinoid species 1,10-phenanthroline-5,6-dione which acted as a proton and electron acceptor in relation to FADH2 cofactor regeneration. Graphite ink was formulated and utilis ed as an underlying conductive layer for LOx enzyme immobilisation and enzymatic polymerisation of 1,10-phenanthroline-5,6-dioneata GC electrode, resulting in a linear range of 0.74 -2.44×10−3 M, sensitivity of4.11×10−4C cm−2 mM−1 and LODof0.06mM.Scanning electron and electrochemical scanning microscopy realised surface characterisation of the enzyme layers.
| Item Type: | Article |
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| Additional Information: | Cite as: Grace Halpin, Karen Herdman, Eithne Dempsey, Electrochemical investigations into enzymatic polymerisation of 1,10-phenanthroline-5,6-dione as a redox mediator for lactate sensing, Sensors and Actuators Reports, Volume 3, 2021, 100032, ISSN 2666-0539, https://doi.org/10.1016/j.snr.2021.100032. |
| Keywords: | Lactic acid; Lactate oxidase; 1,10-Phenanthroline-5,6-dione; Enzymatic polymerisation; Scanning electrochemical microscopy |
| Academic Unit: | Faculty of Science and Engineering > Chemistry Faculty of Science and Engineering > Research Institutes > Human Health Institute |
| Item ID: | 17381 |
| Identification Number: | https://doi.org/10.1016/j.snr.2021.100032 |
| Depositing User: | Eithne Dempsey |
| Date Deposited: | 06 Jul 2023 10:29 |
| Journal or Publication Title: | Sensors and Actuators Reports |
| Publisher: | Science Direct |
| Refereed: | Yes |
| URI: | |
| Use Licence: | This item is available under a Creative Commons Attribution Non Commercial Share Alike Licence (CC BY-NC-SA). Details of this licence are available here |
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