Wu, Luling and Gardiner, Jordan E. and Kumawat, Lokesh K. and Han, Hai-Hao and Guo, Ruiying and Li, Xin and He, Xiao-Peng and Elmes, Robert B. P. and Sedgwick, Adam C. and Bull, Steven D. and James, Tony D. (2019) Coumarin-based fluorescent ‘AND’ logic gate probes for the detection of homocysteine and a chosen biological analyte. RSC Advances, 9 (45). pp. 26425-26428. ISSN 2046-2069

Preview

Download (924kB) | Preview

Abstract

With this research we set out to develop a number of coumarin-based ‘AND’ logic fluorescence probes that were capable of detecting a chosen analyte in the presence of HCys. Probe JEG-CAB was constructed by attaching the ONOO- reactive unit, benzyl boronate ester, to a HCys/Cys reactive fluorescent probe, CAH. Similarly, the core unit CAH was functionalised with the nitroreductase (NTR) reactive p-nitrobenzyl unit to produce probe JEG-CAN. Both, JEG-CAB and JEG-CAN exhibited a significant fluorescence increase when exposed to either HCys and ONOO- (JEG-CAB) or HCys and NTR (JEG-CAN) thus demonstrating their effectiveness to function as AND logic gates for HCys and a chosen analyte

Item Type:	Article
Additional Information:	Cite as: Wu, L., Gardiner, J.E., Kumawat, L.K., Han, H., Guo, R., Li, X., He, X., Elmes, R.B.P., Sedgwick, A.C., Bull, S.D. & James, T.D. 2019, "Coumarin-based fluorescent 'AND' logic gate probes for the detection of homocysteine and a chosen biological analyte", RSC advances, vol. 9, no. 45, pp. 26425-26428.
Keywords:	Chemistry; Coumarin; Fluorescent indicators; Logic circuits;
Academic Unit:	Faculty of Science and Engineering > Chemistry Faculty of Science and Engineering > Research Institutes > Human Health Institute
Item ID:	17383
Identification Number:	https://doi.org/10.1039/c9ra04908h
Depositing User:	Robert Elmes
Date Deposited:	06 Jul 2023 12:14
Journal or Publication Title:	RSC Advances
Publisher:	Royal Society of Chemistry
Refereed:	Yes
URI:	Publisher
Use Licence:	This item is available under a Creative Commons Attribution Non Commercial Share Alike Licence (CC BY-NC-SA). Details of this licence are available here

Repository Staff Only(login required)

Item control page

Downloads

Origin of downloads