Ronan, Rachel and Kshirsagar, Aniket and Rebelo, Ana Lúcia and Sunny, Abbah and Kilcoyne, Michelle and O'Flaherty, Roisin and Rudd, Pauline M. and Schlosser, Gerhard and Saldova, Radka and Pandit, Abhay and McMahon, Siobhan S. (2022) Distinct Glycosylation Responses to Spinal Cord Injury in Regenerative and Nonregenerative Models. Journal of Proteome Research, 21 (6). pp. 1449-1466. ISSN 1535-3893

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Abstract

Traumatic spinal cord injury (SCI) results in disruption of tissue integrity and loss of function. We hypothesize that glycosylation has a role in determining the occurrence of regeneration and that biomaterial treatment can influence this glycosylation response. We investigated the glycosylation response to spinal cord transection in Xenopus laevis and rat. Transected rats received an aligned collagen hydrogel. The response compared regenerative success, regenerative failure, and treatment in an established nonregenerative mammalian system. In a healthy rat spinal cord, ultraperformance liquid chromatography (UPLC) N-glycoprofiling identified complex, hybrid, and oligomannose N-glycans. Following rat SCI, complex and outer-arm fucosylated glycans decreased while oligomannose and hybrid structures increased. Sialic acid was associated with microglia/macrophages following SCI. Treatment with aligned collagen hydrogel had a minimal effect on the glycosylation response. In Xenopus, lectin histochemistry revealed increased levels of N-acetyl-glucosamine (GlcNAc) in premetamorphic animals. The addition of GlcNAc is required for processing complex-type glycans and is a necessary foundation for additional branching. A large increase in sialic acid was observed in nonregenerative animals. This work suggests that glycosylation may influence regenerative success. In particular, loss of complex glycans in rat spinal cord may contribute to regeneration failure. Targeting the glycosylation response may be a promising strategy for future therapies.

Item Type:	Article
Keywords:	spinal cord injury; glycosylation, Xenopus laevis; rat; regeneration; collagen hydroge;
Academic Unit:	Faculty of Science and Engineering > Chemistry Faculty of Science and Engineering > Research Institutes > Human Health Institute
Item ID:	17750
Identification Number:	https://doi.org/10.1021/acs.jproteome.2c00043
Depositing User:	Roisin O'Flaherty
Date Deposited:	25 Oct 2023 14:38
Journal or Publication Title:	Journal of Proteome Research
Publisher:	American Chemical Society
Refereed:	Yes
URI:	Publisher
Use Licence:	This item is available under a Creative Commons Attribution Non Commercial Share Alike Licence (CC BY-NC-SA). Details of this licence are available here

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