Doran, Michelle M. (2016) The Development and Application of Microelectrochemical Sensors for Real-Time Neurochemical Monitoring of Biological Markers of Oxidative Stress. PhD thesis, National University of Ireland Maynooth.

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Abstract

This thesis investigates methods of studying oxidative stress, particularly its role in Parkinson’s disease (PD) with a specific focus on the substrates oxygen (O2), nitric oxide (NO) and superoxide (O2). The primary aim was the in-vitro development and characterisation of a new O2- biosensor suitable for neurochemical monitoring. This thesis also details the neurochemical monitoring of NO and O2 in an animal model of PD utilising the technique of Long Term In-Vivo Electrochemistry (LIVE). Chapter 1 provides an introduction to the brain, biosensors, the chemical reactivity and formation of reactive oxygen species (ROS) and reactive nitrogen species (RNS) in biological systems, and concurrently their role in oxidative stress and the manifestation of PD. Chapter 2 describes the background theory relevant to the research undertaken, while Chapter 3 details the analytical techniques and experimental methods used in the fabrication and characterisation of the various sensor types utilised throughout this research. The results are divided into three chapters. The first of these, Chapter 4 discusses the development and characterisation of the O2- biosensor outlining the various steps undertaken in the optimisation of the biosensor design with respect to O2- sensitivity. Chapter 5 details the interferences in the O2- calibration process and the relevant modifications made to the biosensor in order to minimise these interferences in the in-vivo environment. This chapter also includes further in-vitro characterisation of the O2- biosensor determining the response time, limit of detection (LOD) and the biosensor’s ability to reject endogenous electroactive interferences present in the brain. Chapter 6 focuses on the electrochemical detection of NO and O2 in a reserpine mediated animal model of PD. The specific focus of this chapter is to elucidate the role of oxidative/nitrosative stress in the etiology and pathophysiology of PD. Additionally, this chapter discusses the O2/NO circadian/diurnal changes over any 24 hr period and shows preliminary results of the impact light reversal induces on striatal NO and O2 levels. Finally, Chapter 7 concludes the thesis, discusses the main experimental outcomes of this research and highlights potential future investigations.

Item Type:	Thesis (PhD)
Keywords:	Development and Application; Microelectrochemical Sensors; Real-Time Neurochemical Monitoring; Biological Markers; Oxidative Stress;
Academic Unit:	Faculty of Science and Engineering > Chemistry
Item ID:	18016
Depositing User:	IR eTheses
Date Deposited:	11 Jan 2024 13:47
URI:
Use Licence:	This item is available under a Creative Commons Attribution Non Commercial Share Alike Licence (CC BY-NC-SA). Details of this licence are available here

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