Halpin, Grace and McEntee, Sinead and Dwyer, Catherine and Lawless, Fergal and Dempsey, Eithne (2022) Lactose Biosensor Development and Deployment in Dairy Product Analysis. Journal of the Electrochemical Society, 169 (037528). pp. 1-14. ISSN 0013-4651

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Abstract

Herein, development of an enzymatic biosensor for rapid quantitation of the disaccharide lactose in dairy samples (whey permeates and milk protein isolates (MPI)) is presented. Biosensor fabrication involved a chitosan/enzyme/crosslinker configuration with enzymes glucose oxidase and β-galactosidase at platinum and glassy carbon electrodes. Solution phase mediation was used to lower the operating potential (Eapp = 0.3 V vs Ag/AgCl), minimising any contribution from endogenous background electroactive species. Potential sweep and potentiostatic experiments realised analytical data for the lactose sensor with linear range 5.83 × 10−3 to 1.65 × 10−2 M, sensitivity 9.41 × 10−4 C cm−2 mM−1 and LOD of 1.38 mM. Scanning electrochemical microscopy realised surface characterisation of the enzyme layers with approach curves and redox competition mode imaging achieved over the active enzyme bilayer. Both glucose and lactose sensing was realised in whey permeate sample measuring 23.7 mM lactose, correcting for free glucose contributing signals, with 92.2% correlation with results obtained from the sample certificate of analysis. Solution phase mediation at glassy carbon enzyme electrodes resulted in lactose quantitation in milk protein isolates, measuring 1.16 mM for the MPI 1 (low lactose) and 1.54 mM for the MPI 10 (standard) sample with validation by HPLC analysis.

Item Type:	Article
Keywords:	Lactose; Biosensor; Development; Deployment in Dairy; Product Analysis;
Academic Unit:	Faculty of Science and Engineering > Chemistry Faculty of Science and Engineering > Research Institutes > Human Health Institute
Item ID:	18269
Identification Number:	https://doi.org/10.1149/1945-7111/ac5e41
Depositing User:	Eithne Dempsey
Date Deposited:	13 Mar 2024 10:38
Journal or Publication Title:	Journal of the Electrochemical Society
Publisher:	Electrochemical Society
Refereed:	Yes
URI:	Publisher
Use Licence:	This item is available under a Creative Commons Attribution Non Commercial Share Alike Licence (CC BY-NC-SA). Details of this licence are available here

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