MURAL - Maynooth University Research Archive Library

    Biotelemetric Monitoring of Brain Neurochemistry in Conscious Rats Using Microsensors and Biosensors

    Calia, Giammario and Rocchitta, Gaia and Migheli, Rossana and Puggioni, Giulia and Spissu, Ylenia and Bazzu, Gianfranco and Mazzarello, Vittorio and Lowry, John P. and O'Neill, Robert D. and Desole, Maria S. and Serra, Pier A. (2009) Biotelemetric Monitoring of Brain Neurochemistry in Conscious Rats Using Microsensors and Biosensors. Sensors, 9 (4). pp. 2511-2523. ISSN 1424-8220

    [img] Download (361kB)

    Share your research

    Twitter Facebook LinkedIn GooglePlus Email more...

    Add this article to your Mendeley library


    In this study we present the real-time monitoring of three key brain neurochemical species in conscious rats using implantable amperometric electrodes interfaced to a biotelemetric device. The new system, derived from a previous design, was coupled with carbon-based microsensors and a platinum-based biosensor for the detection of ascorbic acid (AA), O2 and glucose in the striatum of untethered, freely-moving rats. The miniaturized device consisted of a single-supply sensor driver, a current-to-voltage converter, a microcontroller and a miniaturized data transmitter. The redox currents were digitized to digital values by means of an analog-to-digital converter integrated in a peripheral interface controller (PIC), and sent to a personal computer by means of a miniaturized AM transmitter. The electronics were calibrated and tested in vitro under different experimental conditions and exhibited high stability, low power consumption and good linear response in the nanoampere current range. The in-vivo results confirmed previously published observations on striatal AA, oxygen and glucose dynamics recorded in tethered rats. This approach, based on simple and inexpensive components, could be used as a rapid and reliable model for studying the effects of different drugs on brain neurochemical systems.

    Item Type: Article
    Keywords: Biotelemetry; microsensor; biosensor; glucose; oxygen; ascorbic acid;
    Academic Unit: Faculty of Science and Engineering > Chemistry
    Item ID: 2098
    Depositing User: John Lowry
    Date Deposited: 24 Aug 2010 14:33
    Journal or Publication Title: Sensors
    Publisher: Molecular Diversity Preservation International (MDPI)
    Refereed: Yes
    Use Licence: This item is available under a Creative Commons Attribution Non Commercial Share Alike Licence (CC BY-NC-SA). Details of this licence are available here

    Repository Staff Only(login required)

    View Item Item control page


    Downloads per month over past year

    Origin of downloads