Ryle, James P. and Molony, Karen M. and McDonnell, Susan and Naughton, Thomas J. and Sheridan, John T. (2009) Multispectral lensless digital holographic microscope: imaging MCF-7 and MDA-MB-231 cancer cell cultures. Proceedings of SPIE, 7442 (744206). pp. 1-11. ISSN 0277-786X

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Abstract

Digital holography is the process where an object’s phase and amplitude information is retrieved from intensity images obtained using a digital camera (e.g. CCD or CMOS sensor). In-line digital holographic techniques offer full use of the recording device’s sampling bandwidth, unlike off-axis holography where object information is not modulated onto carrier fringes. Reconstructed images are obscured by the linear superposition of the unwanted, out of focus, twin images. In addition to this, speckle noise degrades overall quality of the reconstructed images. The speckle effect is a phenomenon of laser sources used in digital holographic systems. Minimizing the effects due to speckle noise, removal of the twin image and using the full sampling bandwidth of the capture device aids overall reconstructed image quality. Such improvements applied to digital holography can benefit applications such as holographic microscopy where the reconstructed images are obscured with twin image information. Overcoming such problems allows greater flexibility in current image processing techniques, which can be applied to segmenting biological cells (e.g. MCF-7 and MDA-MB- 231) to determine their overall cell density and viability. This could potentially be used to distinguish between apoptotic and necrotic cells in large scale mammalian cell processes, currently the system of choice, within the biopharmaceutical industry.

Item Type:	Article
Additional Information:	Copyright 2009 Society of Photo-Optical Instrumentation Engineers. One print or electronic copy may be made for personal use only. Systematic reproduction and distribution, duplication of any material in this paper for a fee or for commercial purposes, or modification of the content of the paper are prohibited. The authors acknowledge the support of Enterprise Ireland and Science Foundation Ireland. They also thank the Irish Research Council for Science, Engineering and Technology for their support under the national development plan. James Ryle wishes to thank SPIE for supporting conference attendance through the Student Chapter Officer Travel Grant programme. He also acknowledges financial support from UCD’s Seed Funding Scheme.
Keywords:	In-line digital holography; lensless microscope; Gabor microscopy; wavefront reconstruction;
Academic Unit:	Faculty of Science and Engineering > Computer Science
Item ID:	2495
Identification Number:	https://doi.org/10.1117/12.826882
Depositing User:	Thomas Naughton
Date Deposited:	13 Apr 2011 15:46
Journal or Publication Title:	Proceedings of SPIE
Publisher:	SPIE (Society of Photo-optical Instrumentation Engineers)
Refereed:	Yes
Funders:	Enterprise Ireland, Science Foundation Ireland, Irish Research Council for Science, Engineering and Technology, SPIE, UCD (Seed Funding Scheme)
URI:	http://spiedigitallibrary.org/
Use Licence:	This item is available under a Creative Commons Attribution Non Commercial Share Alike Licence (CC BY-NC-SA). Details of this licence are available here

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