Singh, Ishwar , Vyle, Joseph S. and Heaney, Frances (2009) Supporting Information: Fast, Copper-Free Click Chemistry, A Convenient Solid-Phase Approach to Oligonucleotide Conjugation. Documentation. Royal Society of Chemistry.
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Abstract
General experimental
Analytical TLC was performed on precoated (250 μm) silica gel 60 F-254 plates from Merck.
All plates were visualized by UV irradiation, and/or staining with 5% H2SO4 in ethanol
followed by heating. Flash chromatography grade silica gel 60 (230-400 mesh) was obtained
from Merck. Mass analysis was performed on an Ettan MALDI-TOF Pro from Amersham
Biosciences or LASER-TOF LT3 from Scientific Analytical Instruments with 3-
hydroxypicolinic acid or 2,’ 4’, 6’-trihydroxyacetophenone as matrix. The NMR spectra were
obtained at 1H (300 MHz), 13C (75 MHz) and 31P (121 MHz) on a Bruker instrument at 25 ºC.
Chemical shifts are reported in ppm downfield from TMS as standard. HPLC was carried out
using a Gilson instrument equipped with a UV detector and a Nucleosil C18 column (4.0 ×
250 mm) or Phenomenex Clarity. Fluorescence spectra were recorded on a Varian Cary
Eclipse instrument. All other chemical agents were purchased from Aldrich Chemical
Company unless otherwise noted.
Item Type: | Monograph (Documentation) |
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Additional Information: | (c) The Royal Society of Chemistry 2009 |
Keywords: | Copper-Free Click Chemistry; Solid-Phase Approach; Oligonucleotide Conjugation; |
Academic Unit: | Faculty of Science and Engineering > Computer Science |
Item ID: | 2707 |
Depositing User: | Dr. Frances Heaney |
Date Deposited: | 14 Sep 2011 13:52 |
Publisher: | Royal Society of Chemistry |
Related URLs: | |
URI: | https://mural.maynoothuniversity.ie/id/eprint/2707 |
Use Licence: | This item is available under a Creative Commons Attribution Non Commercial Share Alike Licence (CC BY-NC-SA). Details of this licence are available here |
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