Bernhardt, Paul V. and Schenk, Gerhard and Wilson, Gregory J. (2004) Direct Electrochemistry of Porcine Purple Acid Phosphatase (Uteroferrin). Biochemistry, 43. pp. 10387-10392. ISSN 0006-2960

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Abstract

Cyclic voltammetry of the non-heme diiron enzyme porcine purple acid phosphatase (uteroferrin, Uf) has been reported for the first time. Totally reversible one-electron oxidation responses (FeIII-FeII f FeIII-FeIII) are seen both in the absence and in the presence of weak competitive inhibitors phosphate and arsenate, and dissociation constants of these oxoanion complexes formed with uteroferrin in its oxidized state (Ufo) have been determined. The effect of pH on the redox potentials has been investigated in the range 3 < pH < 6.5, enabling acid dissociation constants for Ufo and its phosphate and arsenate complexes to be calculated.

Item Type:	Article
Keywords:	Electrochemistry; Porcine Purple Acid Phosphatase; Uteroferrin;
Academic Unit:	Faculty of Science and Engineering > Chemistry
Item ID:	3694
Depositing User:	Gary Schenk
Date Deposited:	29 May 2012 09:28
Journal or Publication Title:	Biochemistry
Publisher:	American Chemical Society
Refereed:	Yes
URI:	Publisher
Use Licence:	This item is available under a Creative Commons Attribution Non Commercial Share Alike Licence (CC BY-NC-SA). Details of this licence are available here

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