Kealy, John and Commins, Sean
(2009)
Antagonism of glutamate receptors in the CA1 to perirhinal
cortex projection prevents long-term potentiation and
attenuates levels of brain-derived neurotrophic factor.
Brain Research, 1265.
pp. 53-64.
ISSN 0006-8993
Abstract
The CA1 to perirhinal cortex projection is one of multiple hippocampal–neocortical
projections considered to be involved in memory consolidation. This projection has been
shown to sustain long-term potentiation (LTP) following stimulation of CA1. Here we
examined the pharmacological properties underpinning the plasticity observed in this
projection. A stimulating electrode was inserted into the area CA1 and a recording electrode
was inserted into the perirhinal cortex of urethane-anaesthetised Wistar rats. Rats (n=6 in
each drug group) were administered with either saline (0.09%), MK-801 (NMDA antagonist;
0.1 mg/kg) or CNQX (AMPA/kainate antagonist; 1.5 mg/kg). Baseline recordings were made
for 10 min by stimulating area CA1 (0.05 Hz stimulation protocol). High-frequency
stimulation (HFS; 250 Hz) was performed and post-HFS fEPSP recordings were made for
1 h (0.05 Hz, as above). Baseline and post-HFS paired-pulse facilitation (PPF) recordings were
performed across six different interpulse intervals. CA1 and perirhinal cortex tissue samples
were taken from the stimulated and unstimulated hemispheres of each rat brain and
analysed using a brain-derived neurotrophic factor (BDNF) ELISA. Results indicate that LTP
was induced in the saline and MK-801 groups but not in the CNQX group; fEPSPs in the latter
group rapidly returned to baseline levels following a short period of post-tetanic
potentiation. Drug treatment and HFS had no effect on PPF levels. Drug treatment
significantly reduced concentrations of both CA1 and perirhinal BDNF and prevented
stimulation-induced increases in BDNF in CA1. This molecular and electrophysiological
data suggests that LTP in the CA1–perirhinal cortex projection may require activation of
postsynaptic AMPA/kainate receptors in order to sustain LTP.
| Item Type: |
Article
|
| Additional Information: |
The definitive version of this article is available at Kealy J & Commins S (2009) 'Antagonism of glutamate receptors in the CA1 to perirhinal cortex projection prevents long-term potentiation and attenuates levels of brain-derived neurotrophic factor.'. BRAIN RESEARCH, 1265:53 - 61 . DOI: 10.1016/j.brainres.2009.01.067 © 2009 Elsevier B.V. |
| Keywords: |
LTP; Hippocampus; Perirhinal cortex; AMPA;
NMDA; BDNF; |
| Academic Unit: |
Faculty of Science and Engineering > Psychology |
| Item ID: |
5547 |
| Identification Number: |
https://doi.org/10.1016/j.brainres.2009.01.067 |
| Depositing User: |
Dr. Sean Commins
|
| Date Deposited: |
10 Nov 2014 16:55 |
| Journal or Publication Title: |
Brain Research |
| Publisher: |
Elsevier |
| Refereed: |
Yes |
| URI: |
|
| Use Licence: |
This item is available under a Creative Commons Attribution Non Commercial Share Alike Licence (CC BY-NC-SA). Details of this licence are available
here |
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