Kirwan, Annie
(2012)
Characterisation of the role of
human Evolutionary Conserved
Signalling Intermediate in Toll
(ECSIT) in Mitogen Activated Protein
Kinase (MAPK) signalling.
Masters thesis, National University of Ireland Maynooth.
Abstract
Evolutionarily conserved signalling intermediate in Toll pathways
(ECSIT) was identified orginally as a TNF receptor associated factor
6 (TRAF6) interacting partner. The murine homolog mECSIT has
been shown to be involved in NFκB, MAPK, BMP and mitochondrial
signalling. To date there is no work published on the human
homolog, hECSIT. In this thesis, I present data indicating that hECSIT
is involved in NFκB, BMP and MAPK signalling. There is a striking
difference in the role of hECSIT and mECSIT in the activation of
inflammatory transcription factors NFκB, ELK-1 and AP-1; with
mECSIT augmenting their activation and hECSIT having an inhibitory
role. In addition I demonstrate that hECSIT specifically targets the
p42/44 branch of MAPK signalling. Suppression of endogenous
hECSIT results in increased basal and proinflammatory induced
phosphorylation of p42/44 but not JNK or p38. Thus, hECSIT
signalling represents a novel means of regulating p42/44 and its
downstream targets.
Repository Staff Only(login required)
 |
Item control page |
Downloads per month over past year