Govindarajan, Sridhar and McNeil, Calum J. and Lowry, John P. and McMahon, Colm P. and O'Neill, Robert D.
(2013)
Highly selective and stable microdisc biosensors for l-glutamate monitoring.
Sensors and Actuators B, 178.
pp. 606-614.
ISSN 0925-4005
Abstract
Glutamate mediates most of the excitatory synaptic transmission in the brain, and its abnormal regulation
is considered a key factor underlying the appearance and progression of many neurodegenerative
and psychiatric diseases. In this work, a microdisc-based amperometric biosensor for glutamate detection
with highly enhanced selectivity and good stability is proposed. The biosensor utilizes the enzyme
glutamate oxidase which was dip-coated onto 125 um diameter platinum discs. To improve selectivity,
phosphatidylethanolamine was pre-coated prior to enzyme deposition, and electropolymerization
of o-phenylenediamine was performed to entrap the enzyme within a polymer matrix. A variety of coating
configurations were tested in order to optimize biosensor performance. For stability measurements,
biosensors were biased continuously and calibration curves calculated each day for a period of 5–6 days.
The optimized biosensors exhibited very high sensitivity (71
±
1 mA M−1 cm−2), low detection limit of
∼2.5 uM glutamate, selectivity (over 87% against ascorbic acid), very good temporal stability during continuous
use, and a response time of <5 s. These biosensors are therefore good candidates for further
development as devices for continuous monitoring during traumatic brain injury or neurosurgery.
| Item Type: |
Article
|
| Additional Information: |
The definitive published version of this article is available at DOI: 10.1016/j.snb.2012.12.077 |
| Keywords: |
Glutamate; Biosensor; o-Phenylenediamine; Nafion; Selectivity; Stability; |
| Academic Unit: |
Faculty of Science and Engineering > Chemistry |
| Item ID: |
7011 |
| Identification Number: |
https://doi.org/10.1016/j.snb.2012.12.077 |
| Depositing User: |
John Lowry
|
| Date Deposited: |
09 Mar 2016 10:29 |
| Journal or Publication Title: |
Sensors and Actuators B |
| Publisher: |
Elsevier |
| Refereed: |
Yes |
| URI: |
|
| Use Licence: |
This item is available under a Creative Commons Attribution Non Commercial Share Alike Licence (CC BY-NC-SA). Details of this licence are available
here |
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