MURAL - Maynooth University Research Archive Library

    Highly selective and stable microdisc biosensors for l-glutamate monitoring

    Govindarajan, Sridhar and McNeil, Calum J. and Lowry, John P. and McMahon, Colm P. and O'Neill, Robert D. (2013) Highly selective and stable microdisc biosensors for l-glutamate monitoring. Sensors and Actuators B, 178. pp. 606-614. ISSN 0925-4005

    Download (916kB) | Preview

    Share your research

    Twitter Facebook LinkedIn GooglePlus Email more...

    Add this article to your Mendeley library


    Glutamate mediates most of the excitatory synaptic transmission in the brain, and its abnormal regulation is considered a key factor underlying the appearance and progression of many neurodegenerative and psychiatric diseases. In this work, a microdisc-based amperometric biosensor for glutamate detection with highly enhanced selectivity and good stability is proposed. The biosensor utilizes the enzyme glutamate oxidase which was dip-coated onto 125 um diameter platinum discs. To improve selectivity, phosphatidylethanolamine was pre-coated prior to enzyme deposition, and electropolymerization of o-phenylenediamine was performed to entrap the enzyme within a polymer matrix. A variety of coating configurations were tested in order to optimize biosensor performance. For stability measurements, biosensors were biased continuously and calibration curves calculated each day for a period of 5–6 days. The optimized biosensors exhibited very high sensitivity (71 ± 1 mA M−1 cm−2), low detection limit of ∼2.5 uM glutamate, selectivity (over 87% against ascorbic acid), very good temporal stability during continuous use, and a response time of <5 s. These biosensors are therefore good candidates for further development as devices for continuous monitoring during traumatic brain injury or neurosurgery.

    Item Type: Article
    Additional Information: The definitive published version of this article is available at DOI: 10.1016/j.snb.2012.12.077
    Keywords: Glutamate; Biosensor; o-Phenylenediamine; Nafion; Selectivity; Stability;
    Academic Unit: Faculty of Science and Engineering > Chemistry
    Item ID: 7011
    Identification Number:
    Depositing User: John Lowry
    Date Deposited: 09 Mar 2016 10:29
    Journal or Publication Title: Sensors and Actuators B
    Publisher: Elsevier
    Refereed: Yes
    Use Licence: This item is available under a Creative Commons Attribution Non Commercial Share Alike Licence (CC BY-NC-SA). Details of this licence are available here

    Repository Staff Only(login required)

    View Item Item control page


    Downloads per month over past year

    Origin of downloads