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    Simultaneous recording of hippocampal oxygen and glucose in real time using constant potential amperometry in the freely-moving rat

    Kealy, John and Bennett, Rachel and Lowry, John P. (2013) Simultaneous recording of hippocampal oxygen and glucose in real time using constant potential amperometry in the freely-moving rat. Journal of Neuroscience Methods, 215 (1). pp. 110-120.

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    Amperometric sensors for oxygen and glucose allow for real time recording from the brain in freelymoving animals. These sensors have been used to detect activity- and drug-induced changes in metabolism in a number of brain regions but little attention has been given over to the hippocampus despite its importance in cognition and disease. Sensors for oxygen and glucose were co-implanted into the hippocampus and allowed to record for several days. Baseline recordings show that basal concentrations of hippocampal oxygen and glucose are 100.26 ± 5.76 uM and 0.60 ± 0.06 mM respectively. Furthermore, stress-induced changes in neural activity have been shown to significantly alter concentrations of both analytes in the hippocampus. Administration of O2 gas to the animals’ snouts results in significant increases in hippocampal oxygen and glucose and administration of N2 gas results in a significant decrease in hippocampal oxygen. Chloral hydrate-induced anaesthesia causes a significant increase in hippocampal oxygen whereas treatment with the carbonic anhydrase inhibitor acetazolamide significantly increases hippocampal oxygen and glucose. These findings provide real time electrochemical data for the hippocampus which has been previously impossible with traditional methods such as microdialysis or ex vivo analysis. As such, these sensors provide a window into hippocampal function which can be used in conjunction with behavioural and pharmacological interventions to further elucidate the functions and mechanisms of action of the hippocampus in normal and disease states.

    Item Type: Article
    Additional Information: The definitive published version of this article is available at DOI: 10.1016/j.jneumeth.2013.02.016
    Keywords: Sensor; Biosensor; Metabolism; Anaesthesia; Dimethyl sulfoxide; Acetazolamide;
    Academic Unit: Faculty of Science and Engineering > Chemistry
    Item ID: 7012
    Identification Number:
    Depositing User: John Lowry
    Date Deposited: 09 Mar 2016 10:29
    Journal or Publication Title: Journal of Neuroscience Methods
    Publisher: Elsevier
    Refereed: Yes
    Funders: Enterprise Ireland, Higher Education Authority (HEA)
    Use Licence: This item is available under a Creative Commons Attribution Non Commercial Share Alike Licence (CC BY-NC-SA). Details of this licence are available here

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