Mittag, Judith J. and Milani, Silvia and Walsh, Dominic M. and Rädler, Joachim O. and McManus, Jennifer (2015) Simultaneous measurement of a range of particle sizes during Ab1–42 fibrillogenesis quantified using fluorescence correlation spectroscopy. Biochemical and Biophysical Research Communications, 448 (2). pp. 195-199. ISSN 0006-291X

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Abstract

Low molecular weight oligomers of amyloid beta (Aβ) are important drivers of Alzheimer's disease. A decrease in Aβ monomer levels in human cerebrospinal fluid (CSF) is observed in Alzheimers' patients and is a robust biomarker of the disease. It has been suggested that the decrease in monomer levels in CSF is due to the formation of Aβ oligomers. A robust technique capable of identifying Aβ oligomers in CSF is therefore desirable. We have used fluorescence correlation spectroscopy and a five Gaussian distribution model (5GDM) to monitor the aggregation of Aβ1-42 in sodium phosphate buffer and in artificial cerebrospinal fluid (ACSF). In buffer, several different sized components (monomer, oligomers, protofibrils and fibrils) can be identified simultaneously using 5GDM. In ACSF, the faster kinetics of fibrillogenesis leads to the formation of fibrils on very short timescales. This analysis method can also be used to monitor the aggregation of other proteins, nanoparticles or colloids, even in complex biological fluids.

Item Type:	Article
Keywords:	Aggregation; Alzheimer’s disease; Amyloid beta; Fluorescence correlation spectroscopy; Oligomerization; Protein folding disease;
Academic Unit:	Faculty of Science and Engineering > Chemistry
Item ID:	7754
Identification Number:	https://doi.org/10.1016/j.bbrc.2014.04.088
Depositing User:	Jennifer McManus
Date Deposited:	13 Jan 2017 17:02
Journal or Publication Title:	Biochemical and Biophysical Research Communications
Publisher:	Elsevier
Refereed:	Yes
Funders:	Science Foundation Ireland (SFI), European Science Foundation, Deutsche Forschungsgemeinschaft, Foundation for Neurologic Diseases
URI:	http://www.sciencedirect.com
Use Licence:	This item is available under a Creative Commons Attribution Non Commercial Share Alike Licence (CC BY-NC-SA). Details of this licence are available here

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