Zaina, Zainiharyati M. and O'Neill, Robert D. and Lowry, John P. and Pierce, Kenneth W. and Tricklebank, Mark and Dewae, Aidiahmad and Ab Ghani, Sulaiman (2010) Development of an implantable d-serine biosensor for in vivo monitoring using mammalian d-amino acid oxidase on a poly (o-phenylenediamine) and Nafion-modified platinum–iridium disk electrode. Biosensors and Bioelectronics, 25 (6). pp. 1454-1459. ISSN 0956-5663

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Abstract

d-serine has been implicated as a brain messenger, promoting not only neuronal signalling but also synaptic plasticity. Thus, a sensitive tool for d-serine monitoring in brain is required to understand the mechanisms of d-serine release from glia cells. A biosensor for direct fixed potential amperometric monitoring of d-serine incorporating mammalian d-amino acid oxidase (DAAO) immobilized on a Nafion coated poly-ortho-phenylenediamine (PPD) modified Pt–Ir disk electrode was therefore developed. The combined layers of PPD and Nafion enhanced the enzyme activity and biosensor efficiency by ∼2-fold compared with each individual layer. A steady state response time (t90%) of 0.7 ± 0.1 s (n = 8) and limit of detection 20 ± 1 nM (n = 8) were obtained. Cylindrical geometry showed lower sensitivity compared to disk geometry (61 ± 7 μA cm−2 mM−1, (n = 4), R2 = 0.999). Interference by ascorbic acid (AA), the main interference species in the central nervous system and other neurochemical electroactive molecules was negligible. Implantation of the electrode and microinjection of d-serine into rat brain striatal extracellular fluid demonstrated that the electrode was capable of detecting d-serine in brain tissue in vivo.

Item Type:	Article
Keywords:	Microbiosensor; d-serine; d-amino acid oxidase; Implantable; Neurochemical monitoring;
Academic Unit:	Faculty of Science and Engineering > Chemistry
Item ID:	8027
Identification Number:	https://doi.org/10.1016/j.bios.2009.10.049
Depositing User:	John Lowry
Date Deposited:	13 Mar 2017 16:59
Journal or Publication Title:	Biosensors and Bioelectronics
Publisher:	Elsevier
Refereed:	Yes
Funders:	Science Foundation Ireland (SFI), Eli Lilly
URI:	http://www.sciencedirect.com
Use Licence:	This item is available under a Creative Commons Attribution Non Commercial Share Alike Licence (CC BY-NC-SA). Details of this licence are available here

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