Bazzu, Gianfranco and Puggioni, Giulia and Dedola, Sonia and Calia, Giammario and Rocchitta, Gaia and Migheli, Rossana and Desole, Maria S. and Lowry, John P. and O'Neill, Robert D. and Serra, Pier A. (2009) Real-Time Monitoring of Brain Tissue Oxygen Using a Miniaturized Biotelemetric Device Implanted in Freely Moving Rats. Analytical Chemistry, 81 (6). pp. 2235-2241. ISSN 0003-2700

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Abstract

A miniaturized biotelemetric device for the amperometric detection of brain tissue oxygen is presented. The new system, derived from a previous design, has been coupled with a carbon microsensor for the real-time detection of dissolved O(2) in the striatum of freely moving rats. The implantable device consists of a single-supply sensor driver, a current-to-voltage converter, a microcontroller, and a miniaturized data transmitter. The oxygen current is converted to a digital value by means of an analog-to-digital converter integrated in a peripheral interface controller (PIC). The digital data is sent to a personal computer using a six-byte packet protocol by means of a miniaturized 434 MHz amplitude modulation (AM) transmitter. The receiver unit is connected to a personal computer (PC) via a universal serial bus. Custom developed software allows the PC to store and plot received data. The electronics were calibrated and tested in vitro under different experimental conditions and exhibited high stability, low power consumption, and good linear response in the nanoampere current range. The in vivo results confirmed previously published observations on oxygen dynamics in the striatum of freely moving rats. The system serves as a rapid and reliable model for studying the effects of different drugs on brain oxygen and brain blood flow and it is suited to work with direct-reduction sensors or O(2)-consuming biosensors.

Item Type:	Article
Keywords:	Real-Time Monitoring; Brain Tissue; Oxygen; Miniaturized Biotelemetric Device; Rats; Carbon microsensor;
Academic Unit:	Faculty of Science and Engineering > Chemistry
Item ID:	8031
Identification Number:	https://doi.org/10.1021/ac802390f
Depositing User:	John Lowry
Date Deposited:	20 Mar 2017 17:28
Journal or Publication Title:	Analytical Chemistry
Publisher:	American Chemical Society
Refereed:	Yes
URI:	http://pubs.acs.org/
Use Licence:	This item is available under a Creative Commons Attribution Non Commercial Share Alike Licence (CC BY-NC-SA). Details of this licence are available here

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