Brown, Finbar O. and Finnerty, Niall J. and Bolger, Fiachra B. and Millar, Julian and Lowry, John P. (2005) Calibration of NO sensors for In Vivo Voltammetry: Laboratory synthesis of NO and the use of UV-visible spectroscopy in determining stock concentrations. Analytical and Bioanalytical Chemistry, 381 (4). pp. 964-971. ISSN 1618-2642

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Abstract

The increasing scientific interest in nitric oxide (NO) necessitates the development of novel and simple methods of synthesising NO on a laboratory scale. In this study we have refined and developed a method of NO synthesis, using the neutral Griess reagent, which is inexpensive, simple to perform, and provides a reliable method of generating NO gas for in-vivo sensor calibration. The concentration of the generated NO stock solution was determined using UV–visible spectroscopy to be 0.28±0.01 mmol L−1. The level of NO2− contaminant, also determined using spectroscopy, was found to be 0.67±0.21 mmol L−1. However, this is not sufficient to cause any considerable increase in oxidation current when the NO stock solution is used for electrochemical sensor calibration over physiologically relevant concentrations; the NO sensitivity of bare Pt-disk electrodes operating at +900 mV (vs. SCE) was 1.08 nA μmol−1 L, while that for NO2− was 5.9×10−3 nA μmol−1 L. The stability of the NO stock solution was also monitored for up to 2 h after synthesis and 30 min was found to be the time limit within which calibrations should be performed.

Item Type:	Article
Keywords:	Nitric oxide; Laboratory synthesis; UV–visible spectroscopy; In-vivo voltammetry; Electrochemical sensors; Calibrations;
Academic Unit:	Faculty of Science and Engineering > Chemistry
Item ID:	8041
Identification Number:	https://doi.org/10.1007/s00216-004-2964-8
Depositing User:	John Lowry
Date Deposited:	22 Mar 2017 16:47
Journal or Publication Title:	Analytical and Bioanalytical Chemistry
Publisher:	Springer Verlag
Refereed:	Yes
Funders:	Analytical Chemistry Trust Fund (Royal Society of Chemistry, RSC), Engineering and Physical Sciences Research Council (EPSRC) UK, Enterprise Ireland (EI), Health Research Board (HRB), NUI Maynooth
URI:	http://link.springer.com
Use Licence:	This item is available under a Creative Commons Attribution Non Commercial Share Alike Licence (CC BY-NC-SA). Details of this licence are available here

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