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    Brain Tissue Oxygen: In Vivo Monitoring using Carbon Paste Electrodes

    Bolger, Fiachra B. and Lowry, John P. (2005) Brain Tissue Oxygen: In Vivo Monitoring using Carbon Paste Electrodes. Sensors, 5 (11). pp. 473-487. ISSN 1424-8220

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    In this communication we review selected experiments involving the use ofcarbon paste electrodes (CPEs) to monitor and measure brain tissue O2 levels in awakefreely-moving animals. Simultaneous measurements of rCBF were performed using the H2clearance technique. Voltammetric techniques used include both differential pulse (O2) andconstant potential amperometry (rCBF). Mild hypoxia and hyperoxia produced rapidchanges (decrease and increase respectively) in the in vivo O2 signal. Neuronal activation(tail pinch and stimulated grooming) produced similar increases in both O2 and rCBFindicating that CPE O2 currents provide an index of increases in rCBF when such increasesexceed O2 utilization. Saline injection produced a transient increase in the O2 signal whilechloral hydrate produced slower more long-lasting changes that accompanied the behavioralchanges associated with anaesthesia. Acetazolamide increased O2 levels through an increasein rCBF.

    Item Type: Article
    Keywords: In-vivo voltammetry; cerebral blood flow; hypoxia and hyperoxia; neuronal activation; chloral hydrate anaesthesia; acetazolamide;
    Academic Unit: Faculty of Science and Engineering > Chemistry
    Item ID: 8042
    Identification Number:
    Depositing User: John Lowry
    Date Deposited: 22 Mar 2017 16:46
    Journal or Publication Title: Sensors
    Publisher: Molecular Diversity Preservation International (MDPI)
    Refereed: Yes
    Funders: Health Research Board (HRB), Science Foundation Ireland (SFI), Enterprise Ireland (EI), RSC & EPSRC UK, NUI Maynooth
    Use Licence: This item is available under a Creative Commons Attribution Non Commercial Share Alike Licence (CC BY-NC-SA). Details of this licence are available here

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